摘要
以‘六合黄心芹’和‘文图拉’2个芹菜(Apium graveolens)品种为试验材料,从中克隆得到一个编码bZIP转录因子的基因AgbZIP16。序列分析表明2个芹菜品种中AgbZIP16基因的核苷酸序列相同,包含一个长度为1221bp的开放阅读框(ORF),编码406个氨基酸。序列多重比对及进化树分析表明,bZIP转录因子在植物中具有较高的保守性且在同科植物的进化中比较保守,AgbZIP16与同属伞形科的胡萝卜(Daucus carota)bZIP转录因子进化关系最近。蛋白质理化性质分析表明AgbZIP16蛋白的分子质量为43.12 kDa,理论等电点(pI)为6.33,属于亲水性蛋白。AgbZIP16蛋白的二级结构预测显示该蛋白中包含多个α螺旋和无规则卷曲。实时荧光定量PCR(RT-qPCR)结果表明,AgbZIP16基因在芹菜根中表达量最高,在叶柄中最低,呈现显著的组织特异性,同时对高温、低温、干旱和盐胁迫均有响应。‘六合黄心芹’中AgbZIP16的表达水平在逆境处理后均下调,分别在高温、低温、干旱处理24 h和盐处理1 h时达到最低值。‘文图拉’中AgbZIP16的表达水平在高温处理4 h时显著高于对照,为对照的4.92倍;低温、干旱及盐处理下其表达量整体呈现先上升后下降的趋势,在盐处理24 h和干旱处理8 h时表达量显著上调。结果显示多种非生物胁迫可诱导AgbZIP16基因的表达发生变化,且其在不同芹菜品种中的表达响应具有显著差异。本文研究结果将为进一步开展芹菜bZIP类转录因子的逆境调控研究提供参考。
Two celery(Apium graveolens)varieties,‘Liuhe Huangxinqin’and‘Ventura’,were used as experimental materials to clone and obtain the AgbZIP16 gene encoding a bZIP transcription factor.The nucleotide sequences of AgbZIP16 genes of two celery varieties are identical.AgbZIP16 contains an open reading frame(ORF)of 1221 bp and encodes 406 amino acids.Multiple alignments and phylogenetic tree analysis of amino acid sequences indicates that bZIP transcription factors are highly conserved in plants and are relatively conservative in the evolution of the same family.The AgbZIP16 protein has the closest genetic relation with bZIP protein of carrot,which also belongs to Apiaceae.The molecular mass of AgbZIP16 protein is 43.12 kDa;the theoretical isoelectric point is 6.33;it is a hydrophilic protein.Secondary structure prediction shows AgbZIP16 protein possesses multipleα-helices and random coils.The results of quantitative real time PCR(RT-qPCR)show that AgbZIP16 displayed the highest expression level in celery root and the lowest in petiole,which suggests tissue specificity.Moreover,the expression of AgbZIP16 gene was affected by high temperature,low temperature,drought and high-salt conditions.The expression level of AgbZIP16 in‘Liuhe Huangxinqin’decreased under abiotic stress treatments,and reached the minimum when treated by high temperature,low temperature,drought for 24 h and salt treatment for 1 h.They were 24%,2%,3%,and 24%of the control,respectively.The expression profiles of AgbZIP16 in‘Ventura’were also detected.It was significantly higher than that of the control at 4 h under high temperature treatment,which was 4.92 times higher than that of the control,and the gene expression didn’t show significant difference at other time points.Under the low temperature,drought and salt treatments,AgbZIP16 gene expression first increased and then decreased.The expression levels of AgbZIP16 gene were significant up-regulated at 24 h of salt treatment and 8 h of drought treatment.These results indicate that various abiotic stresses could induce the changes in the expression of AgbZIP16 gene.The expression response also varied in different celery cultivars.The results would provide a potential foundation for further research on the regulation roles in abiotic stress of the member of bZIP transcription factor family in celery.
作者
沈迪
陈龙正
陶建平
刘洁霞
冯凯
尹莲
徐志胜
熊爱生
SHEN Di;CHEN Long-Zheng;TAO Jian-Ping;LIU Jie-Xia;FENG Kai;YIN Lian;XU Zhi-Sheng;XIONG Ai-Sheng(State Key Laboratory of Crop Genetics and Germplasm Enhancement,Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;Institute of Vegetable,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Institute of Agricultural Facilities and Equipment,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2019年第12期1817-1826,共10页
Plant Physiology Journal
基金
江苏省农业自主创新资金项目[CX(18)2007]
国家自然科学基金(31272175)
教育部新世纪优秀人才支持计划项目(NCET-11-0670)
江苏高校优势学科建设项目(PAPD).
