摘要
本研究对分离自黑龙江省马铃薯中的1个线虫群体Hp1进行形态观察与测量,采用线虫通用引物对其rDNA-ITS进行扩增与序列分析,利用PAUP软件以ML法构建系统发育树,并测定其致病性。结果显示,该线虫群体有6条侧线,头部略缢缩,口针明显,中食道球呈长纺锤形,食道峡部窄而细长;雌虫阴门稍突起,后阴子宫囊较长;雄虫交合刺略向腹面弯曲,引带短,具交合伞。其形态测量值与腐烂茎线虫(Ditylenchus destructor)基本一致。rDNA-ITS PCR扩增片段916 bp,经BLAST比对,该ITS序列与腐烂茎线虫序列相似度最高。系统发育树显示,该群体没有与腐烂茎线虫A、B基因型群体聚在一起,而是与C、D型群体聚为1个分支,且与C型亲缘关系更近。据此,将群体Hp1鉴定为腐烂茎线虫C基因型,这是首次在黑龙江省发现腐烂线虫为害马铃薯。
The morphological characteristics of a nematode population Hp1 isolated from potato in Heilongjiang were observed and measured in this experiment.The rDNA-ITS of the population was amplified using the universal primers and analyzed and the phylogenetic tree was constructed by ML method of PAUP software.The results showed that Hp1 had 6 lateral lines with slightly constricted head,obvious stylet,oval median bulb and narrow and elongated isthmus of the esophagus.The vulva of female slightly raised with long post-uterine sac.The spi-cule of the male was slightly ventral with short gubernaculum and a bursa.The measurements were largely consistent with Ditylenchus destructor.The amplified product of rDNA-ITS was 916 bp and the sequence shared highest similarity with D.destructor.The population Hp1 was not clustered with type A and B,but clustered into one branch with population of haplotype C and D,and had closer affinity with the type C.Hereby,nematode population Hp1 from the potato was confirmed as haplotype C of D.destructor and this is the first report in Heilongjiang.
作者
张淑玲
李惠霞
徐鹏刚
刘永刚
于洪涛
刘向
ZHANG Shu-ling;LI Hui-xia;XU Peng-gang;LIU Yong-gang;YU Hong-tao;LIU Xiang(College of Plant Protection of Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,Lanzhou 730070,China;Institute of Plant Protection,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China;Suihua Branch,Heilongjiang Academy of Agricultural Sciences,Suihua 152052,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2019年第6期756-762,共7页
Acta Phytopathologica Sinica
基金
国家自然科学基金(31760507)
甘肃农业大学学科建设基金(GAU-XKJS-2018-146).
