摘要
目的:建立UHPLC同时测定显脉旋覆花花序中7个成分(绿原酸,3,5-二咖啡酰奎宁酸,1,5-二咖啡酰奎宁酸,山柰酚-3-O-芸香糖苷,槲皮素,山柰酚,百里香酚)含量,为其质量控制提供参考。方法:采用Agilent Poroshell 120 C18色谱柱(3.0 mm×100 mm,2.7μm),流动相0.1%甲酸水溶液(A)-甲醇(B)梯度洗脱(0~4 min,2%B;4~6 min,2%~5%B;6~10 min,5%~10%B;10~20 min,10%~20%B;20~30 min,20%~27%B;30~37 min,27%~25%B;37~45 min,25%~32%B;45~68 min,32%~58%B;68~75 min,58%~25%B;75~82 min,25%~2%B;82~90 min,2%B),流速0.3 m L·min-1,检测波长254 nm。结果:7个成分在考察的浓度范围内,浓度与峰面积之间呈良好的线性关系(r>0.999);回收率均在97.80%~101.28%,RSD均≤3.0%,SPSS软件的聚类分析和SIMCA软件的主成分分析能较好地把4个不同产地的样品进行分类。结论:该方法简便、准确,可用于不同比较不同来源显脉旋覆花花序的差异性及其质量控制。
Objective:To establish an ultra-high-performance liquid chromatography(UHPLC)method for simultaneous determination of seven components(chlorogenic acid,3,5-dicaffeoylquinic acid,1,5-dicaffeoylquinic acid,kaempferol-3-O-rutinoside,quercetin,kaempferol and thymol)in blossoms of Inula nervosa,and provide references for its quality control.Method:The separation was performed on an Agilent Poroshell 120 C18 column(3.0 mm×100 mm,2.7μm)with 0.1%formic acid(A)and methyl(B)as mobile phase for gradient elution(0-4 min,2%B;4-6 min,2%-5%B;6-10 min,5%-10%B;10-20 min,10%-20%B;20-30 min,20%-27%B;30-37 min,27%-25%B,37-45 min,25%-32%B;45-68 min,32%-58%B;68-75 min,58%-25%B;75-82 min,25%-2%B;82-90 min,2%B).The flow rate was 0.3 m L·min-1 and the detection wavelength was 254 nm.Result:There was a good linear relationship between the concentration and peak area of all the seven components in the investigated concentration range(r>0.999).The average recoveries ranged from 97.80%to 101.28%with RSD≤3.0%.Cluster analysis of SPSS software and principal component analysis of SIMCA software can be used to intuitively classify samples from four different origins.Conclusion:The established method is simple and fast with high precision,which can be used to compare the differences of blossoms of Inula nervosa from different origins and efficiently control its quality.
作者
温芝琪
吴东
龚明
龚建平
黄文平
WEN Zhi-qi;WU Dong;GONG Ming;GONG Jian-ping;HUANG Wen-ping(Jiangxi University of Traditional Chinese Medicine(TCM),Nanchang 330004,China;Solid Preparation of TCM Manufacturing Technology National Engineering Center,Nanchang 330006,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2020年第7期135-140,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重点研发计划项目(2017YFC1702906)
江西省卫计委中医药科研项目(2018B144).
