摘要
目的:建立傣药白花臭牡丹根中总黄酮的含量测定方法。方法:采用分光光度法通过线性关系、准确度、精密度、不同产地以及试验条件的考察建立傣药白花臭牡丹根中总黄酮的含量测定方法,并测定3个不同产地10批傣药白花臭牡丹根中总黄酮的含量。结果:检测波长为357 nm,重复性试验的相对标准偏差为0.16%,回收率为94.907%~101.976%,标准品芦丁在范围内线性关系良好,r^(2)=0.99985。10批样品总黄酮的含量分别为7.62%、7.08%、6.41%、5.93%、6.34%、6.61%、6.50%、6.54%、6.98%、6.27%。不同产地的样品中总黄酮的含量差异不明显。结论:该方法简便、快速、准确,适用于傣药白花臭牡丹根的日常分析和质量控制。
Objective:To establish the method for content determination of total flavonoids in Clerodendrum philippinum Schau.var.simplex C.Method:UV spectrophotometry was used with analysis the linearity,repeatability,precision and recovery rate,different areas and test conditions to establish the total flavonoids determination;10 Samples form 3 different areas were detected.Results:Detective wavelength was 357 nm,the RSD of repeatability was 0.16%,the recovery rate range was 94.907%~101.976%,the linear in the range of 2~46μg/mL,r^(2)=0.99985.The results of 10 samples were 7.62%、7.08%、6.41%、5.93%、6.34%、6.61%、6.50%、6.54%、6.98%、6.27%respectively.Conclusion:The method was simple,rapid,accurate and suitable for routine analysis and quality control of Clerodendrum philippinum Schau.var.simplex C.
作者
李清
陆应彩
周林
彭霞
LI Qing;LU Ying-cai;ZHOU Lin;PENG Xia(Xishuangbanna Institute For Food and Drug Control,Jinhong 666100,China)
出处
《中国民族医药杂志》
2022年第6期56-58,共3页
Journal of Medicine and Pharmacy of Chinese Minorities
基金
傣药材和饮片标准研究制定项目(2019-YZ-065)
关键词
紫外分光光度法
白花臭牡丹
总黄酮
含量测定
UV Spectrophotometry
Clerodendrum philippinum Schau.var.simplex C.
Total Flavonoids
Content Determination
