摘要
目的:以土庄绣线菊为原料提取茶多酚,优化提取条件并研究其抗氧化活性。方法:比较料液比、浸提时间和浸提溶剂对土庄绣线菊茶多酚提取率的影响,确定提取最佳工艺参数。采用铁氰化钾还原法测定茶多酚的总还原能力,以邻苯三酚自氧化法测定其对超氧阴离子自由基的清除作用、DPPH自由基清除率和对Fe^2+络合能力来评价其抗氧化能力。结果:土庄绣线菊料液比1︰20(g/mL),浸提温度70℃,乙醇体积分数70%,浸提时间20 min时提取的茶多酚含量平均为50.9 mg/g。茶多酚具有显著的还原能力和对Fe^2+的络合能力;对O2-·和DPPH自由基清除作用相对较弱,其半数抑制浓度(IC50)分别为0.520 mg/mL和0.376 mg/mL。结论:土庄绣线菊中茶多酚含量丰富,且抗氧化活性较强,可作为潜在天然抗氧化剂应用于食品和医药工业。
Objective:To optimize extraction conditions,extract tea polyphenols from Spiraea pubescens Turcz.,and to study its antioxidant activity.Methods:The effects of material-liquid ratio,extraction time and extraction solvent on the extraction rate of tea polyphenols from Spiraea pubescens Turcz.were evaluated.After that,the best parameters of extraction were determined.The total reducing capacity of tea polyphenols was determined by pyrogallol autoxidation.The total antioxidant activity of tea polyphenols from Spiraea pubescens Turcz.was determined by the scavenging rate of DPPH radical and the complexing capacity of Fe^2+.Results:The optimal conditions were material liquid ratio of 1∶20(g/mL),extraction temperature of 70℃,ethanol volume fraction of 70%and extraction time of 20 min.The content of tea polyphenols extracted was 50.9 mg/g.Tea polyphenols had high reducing capacity and strong complexation ability on Fe^2+,but weak scavenging effect on O2-·and DPPH free radicals,and half of its suppression rate(IC50)was 0.520 mg/mL and 0.376 mg/mL,respectively.Conclusion:Tea polyphenols were abundant in Spiraea pubescens Turcz.and exhibited strong antioxidant activity.It can be used as a potential natural antioxidant in food and pharmaceutical industry.
作者
杨洋
李珍
杜之平
丁华
Yang Yang;Li Zhen;Du Zhiping;Ding Hua(Ordos Inspection&Research Center for Drug and Medical Devices,Ordos 017000,China)
出处
《中国药事》
CAS
2020年第3期342-348,共7页
Chinese Pharmaceutical Affairs
基金
内蒙古自治区标准化项目《53种蒙药材和9种炮制品的标准研究》(编号2016-[MB02]-E04)
鄂尔多斯市应用技术研究与开发资金项目(编号2019-501).
关键词
土庄绣线菊
茶多酚
抗氧化
自由基
还原力
Spiraea pubescens Turcz.
teapolyphenols
antioxidant acitivity
free radicals
reducing power
