摘要
为了探究已经开展净化的广西地方品种鸡中禽白血病病毒(Avian leukosis virus,ALV)分离株的遗传多样性,通过病毒分离后细胞培养上清ALV群特异性抗原p27的ELISA检测和细胞培养物病毒亚群特异性PCR鉴定。结果显示:分离到2株J亚群ALV(ALV-J),分别命名为GX19LZ191J和GX19YL53J,2株均属于Clade 1.3分支;病毒囊膜蛋白ENV的序列比对分析显示,2株在氨基酸残基第192~194位具有一个潜在的N连接的糖基化位点(NLS);Alpha-Flod2同源建模发现,这个潜在的N连接的糖基化位点在二级结构的一个α-螺旋上(氨基酸残基第190~200位)。研究结果表明,广西地区属于Clade 1.3的ALV-J分离株增多,且需重点关注分离株ENV蛋白α-螺旋区域增加一个潜在的N连接的糖基化位点对致病性的影响。
In order to explore the genetic diversity of avian leukosis virus(ALV)isolates in Guangxi local Yellow-chickenthat has been carried out the eradication,a series of laboratory tests were conducted,including ELISA detection of ALV group-specific antigen p27 in DF-1 cell culture supernatants infected with the samples after virus isolation and the identification ofALV subgroup-specific PCR on the cell cultures.The results showed that two isolates of J subgroup ALV(ALV-J)were isolatedfrom the cell cultures,named GX19 LZ191 J and GX19 YL53 J,respectively.Two isolates all belonged to Clade 1.3 ALV-J.Basedon the sequence alignment analysis of the viral envelope protein(ENV),two isolates carried a potential N-linked glycosylationsite(NLS)at residuals 192 to 194.Protein homology modeling used Alpha Flod2 technology showed that the potential NLS was lo-cated in one of theα-helix domains of the secondary structure(at residues 190 to 200).The results indicated that the number ofALV-J isolates belonging to Clade 1.3 increased in Guangxi,and it was necessary to focus on the effect of the addition of a poten-tial NLS inα-helix region of viral ENV protein of the isolates on pathogenicity.
作者
李秋红
朱玮钰
付福梅
杨耀焱
郭金晗
邓乔木
韦平
LI Qiuhong;ZHU Weiyu;FU Fumei;YANG Yaoyan;GUO Jinhan;DENG Qiaomu;WEI Ping(Institute of Poultry Science and Health,Guangxi University,Nanning,Guangxi 530005)
出处
《中国家禽》
北大核心
2022年第6期36-41,共6页
China Poultry
基金
国家现代农业产业技术体系广西肉鸡创新团队建设专项(nycytxgxcxtd-19-03)
