摘要
目的探讨松节方醇提物(SJF)对炎症动物模型T细胞抗原受体(TCR)信号通路酪氨酸激酶及磷酸酶相关分子表达的作用机制。方法采用类风湿关节炎(RA)大鼠模型,给予SJF 2周,酶联免疫吸附法(ELISA)检测RA大鼠关节液中蛋白酪氨酸激酶(Lck、Fyn、ZAP70)、酪氨酸磷酸酶(CD45)含量,观察RA大鼠踝关节组织病理学变化,免疫组化检测RA大鼠踝关节滑膜组织中Lck、Fyn、ZAP70、CD45表达情况。结果 SJF能显著降低RA大鼠关节液Lck、Fyn、ZAP70、CD45含量,抑制RA大鼠滑膜组织炎性细胞浸润和滑膜组织增生,同时明显下调滑膜组织Lck、Fyn、ZAP70、CD45的表达。结论 SJF对RA大鼠的作用机制可能是通过抑制异常激活的TCR信号通路酪氨酸激酶及磷酸酶相关分子的表达来调控其下游的各级信号通路转导功能,从而起到抗炎作用。
Objective To investigate the effectof ethanol extract of Songjie Fang(SJF) effect on the expression of tyrosine kinase and phosphatase-related molecules in the T cell antigen receptor(TCR) signaling pathway in rheumatoid arthritis rats and its mechanism. Methods The rheumatoid arthritis(RA) rat model was administered SJF for 2 weeks. Then the content of tyrosine kinase(Lck, Fyn, ZAP70) and tyrosine phosphatase(CD45) in joint fluids was detected by enzyme-linked immunosorbent assay(ELISA). To observe the histopathological changes of the ankle joints, and the expressions of Lck, Fyn, ZAP70 and CD45 in the synovial tissue were detected by immunohistochemistry. Results The ethanol extract of SJF significantly reduced the content of Lck, Fyn, ZAP70 and CD45 in joint fluid, inhibited the infiltration of inflammatory cells and hyperplasia in the synovial tissue. Meanwhile, down-regulated the expression of Lck, Fyn, ZAP70 and CD45 in synovial tissue significantly. Conclusion The mechanism of anti-inflammation affected by ethanol extract of SFJ may be related to inhibiting the tyrosine kinase and phosphatase-related molecules in the TCR signaling pathway.
作者
莫宗成
李恒华
黄文涛
吴思澜
梅小利
兰波
黄崇刚
MO Zong-cheng;LI Heng-hua;HUANG Wen-tao;WU Si-lan;MEI Xiao-li;LAN Bo;HUANG Chong-gang(Three Grade Laboratory of Pharmacology of Chinese Medicine of Chinese Medicine Management Bureau,Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China)
出处
《中草药》
CAS
CSCD
北大核心
2020年第2期406-411,共6页
Chinese Traditional and Herbal Drugs
基金
重庆市科委基本业务费项目(cstc2018jxjl-jbky130009)
重庆市卫计委中医药科技项目(ZY201802123).
