摘要
[目的]探讨初级纤毛相关基因KIF3A通过Hedgehog信号通路对口腔鳞状细胞癌增殖能力产生的影响.[方法]选择人舌鳞癌Tca8113细胞株作为研究对象,分为KIF3A-siRNA转染组和对照组.采用蛋白印迹实验和实时荧光定量实验检测各组KIF3A、Shh、Gli1蛋白及mRNA的相对表达水平;利用MTT细胞增殖实验观察Tca8113细胞增殖能力的变化.[结果]蛋白印迹实验结果显示,与对照组比较,KIF3A-siRNA转染组KIF3A、Shh、Gli1蛋白表达水平均明显下调(P<0.05);实时荧光定量实验结果显示,与对照组比较,KIF3A-siRNA转染组KIF3A、Shh、Gli1 mRNA表达水平均明显下调(P<0.01);MTT细胞增殖实验结果显示,细胞培养24 h时两组细胞增殖间差异无统计学意义(P>0.05),细胞培养48、72 h时KIF3A-siRNA转染组增殖率较对照组明显降低(P<0.01).[结论]沉默初级纤毛相关基因KIF3A可能通过调控Hedgehog信号通路抑制口腔鳞癌的增殖能力.
OBJECTIVE To explore the effect of primary cilium-associated gene KIF3 A on oral squamous cell carcinoma proliferation through Hedgehog signaling pathway.METHODS Human tongue squamous cell carcinoma Tca8113 cell line was selected and divided into KIF3 A-siRNA transfection group and control group.Western blot and real-time qPCR were used to detect the relative expression levels of KIF3 A,Shh and Gli1 proteins and mRNA in each group.MTT cell proliferation assay was used to observe the proliferation of Tca8113 cells.RESULTS Western blot results showed that compared with the control group,the protein expression levels of KIF3 A,Shh and Gli1 in the KIF3 A-SiRNA transfection group were significantly down-regulated(P<0.05).Real-time qPCR results showed that compared with the control group,the mRNA expression levels of KIF3 A,Shh and Gli1 in the KIF3 A-SiRNA transfection group were significantly down-regulated(P<0.01).MTT cell proliferation assay showed that there was no significant difference in cell proliferation between the two groups after 24 h cell culture(P>0.05).The proliferation rate of the KIF3 A-SiRNA transfection group was significantly lower than that of the control group after cell culture for 48 and 72 h(P<0.01).CONCLUSION Silencing primary cilium-associated gene KIF3 A may inhibit the proliferation of oral squamous cell carcinoma by regulating Hedgehog signaling pathway.
作者
黄银珠
李京旭
金成日
HUANG Yinzhu;LI Jingxu;JIN Chengri(Department of Stomatology,Affiliated Hospital of Yanbian University,Yanji 133000,Jilin,China)
出处
《延边大学医学学报》
CAS
2022年第1期7-10,共4页
Journal of Medical Science Yanbian University
基金
国家自然科学基金项目(编号:81560179)
