摘要
目的分析采用原子荧光(AFS)法和电感耦合等离子体-质谱(ICP-MS)法分别测定尿中砷水平的差异与原因。方法将样品按照WS/T 474—2015《尿中砷的测定氢化物发生原子荧光法》进行前处理,并采用混合酸(硝酸∶硫酸∶高氯酸体积比为3∶1∶1)消解后,分别采用AFS法和ICP-MS法进行测定;另取样品以体积分数为0.50%的硝酸稀释后,采用ICP-MS法测定。样品包括尿中砷元素质量控制样品、无机砷和有机砷(砷胆碱和砷甜菜碱)的加标样品。结果采用AFS法(消解)和ICP-MS法(不消解)对质量控制样品的测定结果均在参考值范围内,但AFS法测得值低于ICP-MS法。2种方法测得的尿中无机砷加标回收率分别为97.79%~100.82%和99.55%~99.98%。AFS法测得中、高质量浓度组无机砷水平均低于ICP-MS法(P值均<0.01)。对于砷甜菜碱和砷胆碱,AFS法(消解)加标回收率仅为2.33%~2.61%,测得值分别低于ICP-MS法(不消解)和ICP-MS法(消解)(P值均<0.01)。结论采用AFS法测得的尿中砷结果低于ICP-MS法,这可能与AFS法配套的混合酸消解方法有关。
Objective To compare the measured results of arsenic in urine by atomic fluorescence spectrometry(AFS)and inductively coupled plasma-mass spectroscopy(ICP-MS),and analyze the reasons of the difference.Methods The samples were pretreated according to WS/T 474-2015 Determination of Arsenic in Urine by Hydride Generation Atomic Fluorescence Spectrometry,and digested with mixed acid(nitric acid∶sulfuric acid∶perchloric acid=3∶1∶1,V/V/V)and then determined by AFS and ICP-MS.The samples were diluted with 0.50%nitric acid and determined by ICP-MS.The samples included urine arsenic quality control samples,inorganic arsenic supplemented samples,and organic arsenic(arsenic choline and arsenic betaine)supplemented samples.Standard curve method was used to compare the results of AFS method and ICP-MS method.Results The results of quality control samples by AFS method(digestion)and ICP-MS method(without digestion)were within the range of reference values,but the values obtained by AFS method were lower than those obtained by ICP-MS method.The recovery of AFS and ICP-MS was 97.79%-100.82%and 99.55%-99.98%,respectively.In the middle and high concentration groups,the measured values of inorganic arsenic by AFS were lower than that by ICP-MS(all P<0.01).The recovery of arsenic betaine and arsenic choline by AFS method(digestion)was only 2.17%-2.63%.The values of arsenic betaine and arsenic choline measured by AFS method(digestion)were lower than those measured by ICP-MS method(without digestion)and ICP-MS method(digestion)(all P<0.01).Conclusion The result of urine arsenic measured by AFS method was lower than that measured by ICP-MS method,which may be related to the mixed acid digestion of AFS method.
作者
梁嘉斌
何易楠
高云霞
郭嘉明
郭尧平
陈纠
刘移民
王致
LIANG Jia-bin;HE Yi-nan;GAO Yun-xia;GUO Jia-ming;GUO Yao-ping;CHEN Jiu;LIU Yi-min;WANG Zhi(Guangzhou Twelfth People's Hospital,Guangzhou,Guangdong 510620,China;不详)
出处
《中国职业医学》
CAS
北大核心
2022年第4期421-424,共4页
China Occupational Medicine
基金
广东省医学科学技术研究基金(A2019426)
广州市科技计划项目(202201010816)
广州市卫生健康科技重大项目(2021A031003)
广州市医学重点学科(2021-2023)
关键词
原子荧光法
电感耦合等离子体-质谱
尿
砷
无机砷
砷甜菜碱
砷胆碱
Atomic fluorescence spectrometry
ICP-MS
Urine
Arsenic
Inorganic arsenic
Arsenic betaine
Arsenic choline
