摘要
目的通过对癌症基因组图谱(TCGA)数据库中胃腺癌转录组数据进行分析,找出预后相关的差异表达基因。方法从TCGA数据库中下载胃腺癌的转录组数据,利用R语言edgeR包筛选出差异基因后,对差异基因进行生存分析。结果共得到175个差异表达miRNA及201个差异表达mRNA/lncRNA,进一部进行生存分析,以P<0.05作为统计筛选标准,得到15个miRNA和9个mRNA、8个lncRNA与胃癌的生存时间相关。结论通过对TCGA数据库中胃腺癌的表达数据进行分析研究,选出与该病预后密切相关的基因,为该病的早期诊断和靶向治疗提供分子层面的参考信息。
Objective To analyze the differentially expressed genes related to prognosis by analyzing the transcriptome data of gastric adenocarcinoma in The Cancer Genome Atlas(TCGA)database.Methods The transcriptome data of gastric adenocarcinoma were downloaded from the TCGA database.After using the edgeR package based on the R language to screen out the differential genes,and analyze the differential genes for predicting survival.Results A total of 175 differentially expressed miRNAs and 201 differentially expressed mRNA/lncRNA were obtained after the survival analysis.For survival analysis,P<0.05 was used as the statistical screening standard.15 miRNA,9 miRNA,and 8 lncRNA were associated with the survival time of gastric cancer.Conclusion By analyzing the expression data of gastric adenocarcinoma in TCGA database,the genes had significant influence on the prognosis of gastric cancer patients,and may provide molecular information for early diagnosis and targeted therapy.
作者
田如月
郭水龙
曹邦伟
TIAN Ru-yue;GUO Shui-long;CAO Bang-wei(Oncology Center,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Department of Gastroenterology,Beijing Friendship Hospital,Capital Medical University,National Clinical Research Center for Digestive Disease,Beijing Digestive Disease Center,Beijing Key Laboratory for Precancerous Lesion of Digestive Disease,Beijing 100050,China)
出处
《临床和实验医学杂志》
2020年第5期449-452,共4页
Journal of Clinical and Experimental Medicine
基金
国家自然科学基金(编号:81670474)
北京市自然科学基金(编号:7172061).
关键词
胃癌
预后
生物标志物
Gastric cancer
Prognosis
Biomarkers
