摘要
以耐药大肠杆菌(M13)和敏感质控菌株(E.coli ATCC25922)为研究对象,探究纳米TiO2和四环素对大肠杆菌生长的影响。结果表明,与单独添加梯度浓度四环素相比,纳米TiO2的加入进一步抑制了敏感菌的生长,而耐药菌变化不大。纳米TiO2单独和联合暴露下对耐药菌的抑菌率均在40%以下,而敏感菌在80%-100%之间,耐药菌M13除了耐抗生素,对纳米TiO2也存在抗性。微生物对抗生素和其它环境毒性因子的抗性机制存在着一些共性,但在共暴露条件下微生物的抗性机制还有待进一步研究。
The drug-resistant Escherichia coli(M13)and sensitive quality control strain(E.coli ATCC25922)were used to study the effects of nano-TiO2 and tetracycline on the growth of E.coli.The results showed that the addition of nano-TiO2 further inhibited the growth of sensitive bacteria compared with the addition of the gradient concentration of tetracycline alone,while the drug-resistant bacteria did not change much.The antibacterial rate of nano-TiO2 alone or co-exposure to the drug-resistant bacteria was below 40%,while that to the sensitive bacteria ranged in 80%and 100%.The drug-resistant strain M13 was resistant to antibiotics and also to nano-TiO2.There are some commonalities between microbial resistance mechanisms against antibiotics and other environmental virulence factors,but the mechanism of microbial resistance under co-exposure conditions remains to be further studied.
作者
张永丽
袁伟
杨清香
ZHANG Yong-li;YUAN Wei;YANG Qing-xiang(College of Life Sciences,Henan Normal University,Xinxiang 453007)
出处
《生物技术通报》
CAS
CSCD
北大核心
2019年第11期124-131,共8页
Biotechnology Bulletin
基金
国家自然科学基金项目(21477035,21277041).
关键词
耐药大肠杆菌
纳米二氧化钛
四环素
抑菌率
drug-resistant Escherichia coli
nano-TiO2
tetracycline
antibacterial rate
