摘要
以藜麦为原料,藜麦甾醇得率为考察指标,利用超临界CO_(2)-皂化法萃取藜麦甾醇,通过单因素和正交试验确定最佳提取工艺条件,利用气相色谱串联质谱法(gas chromatography-mass spectrometer,GC-MS)对藜麦油脂进行成分分析。结果表明,提取藜麦甾醇的最佳条件为:萃取压力25 MPa、萃取温度50℃、萃取时间2.0 h,在此条件下,藜麦甾醇的得率可达20.20 mg/g。从藜麦油脂中共分离鉴定出29种脂肪酸,其中主要的脂肪酸为亚油酸、亚麻酸和油酸,不饱和脂肪酸的含量为74.59%,超长链脂肪酸含量高达27.66%,是其它品种藜麦含量的2倍;支链脂肪酸的含量达0.63%。该研究为藜麦的深度开发和质量评价提供理论依据。
Using quinoa as a raw material and the quinoa sterol yield as a test index,quinoa sterols were extracted by a supercritical CO_(2)-saponification method involving a single factor and an orthogonal experiment,and the components of quinoa oil were analyzed by gas chromatography-mass spectrometry.The results demonstrate that the optimal conditions were extraction pressure of 25 MPa,extraction temperature of 50℃,and extraction time of 2.0 h.Under the optimized conditions,the quinoa oil yield was 20.20 mg/g.A total of 29 fatty acids were isolated from the quinoa oil and identified,with the major components being:linoleic acid,linolenic acid,and oleic acid.The unsaturated fatty acid content was 74.59%,while very long-chain fatty acids accounted for 27.66%,which was twice that of other quinoa varieties;branched-chain fatty acids comprised 0.63%.This study provides a theoretical basis for the further development and quality evaluation of quinoa.
作者
刘雅谦
李琳
孙万成
罗毅皓
LIU Ya-qian;LI Lin;SUN Wan-cheng;LUO Yi-hao(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,Qinghai,China)
出处
《食品研究与开发》
CAS
北大核心
2021年第15期145-151,共7页
Food Research and Development
关键词
藜麦
甾醇
提取
超临界CO_(2)-皂化法
脂肪酸
quinoa
sterol
extraction
supercritical CO_(2)-saponification extraction
fatty acid
