摘要
疫苗是预防百日咳(pertussis)的最有效措施,但无细胞百日咳疫苗接种后免疫力的迅速衰退是近年来百日咳发病率上升的原因之一。百日咳毒素(pertussis toxin,PT)是由百日咳鲍特菌(Bordetella pertussis)分泌的主要毒力因子,具有多种生物学活性。在无细胞百日咳疫苗设计初期,为确保疫苗的有效性,抗PT的单克隆抗体(单抗)常被用来检测PT表面重要的抗原表位。研究表明,与天然PT特异性结合的部分单抗无法识别经化学方法脱毒后的PT,提示化学脱毒会改变疫苗中PT的一些抗原表位,进而影响疫苗保护效果。现结合单抗可用来研究抗体干扰PT功能的分子机制,对机体在感染百日咳后的保护性免疫机制进行综述,以期为无细胞百日咳疫苗的进一步改进及开发人源化抗PT单抗治疗百日咳提供科学依据。
Vaccinations are the most effective measures to prevent pertussis.However,one major reason for the recent recurrence of pertussis is because of the rapid waning immunity after vaccinations with acellular pertussis vaccine(aPV).Pertussis toxin(PT),the causative agent of pertussis,is a major virulence factor of Bordetella pertussis and has various biological activities.In the early stage of aPV design,anti-PT monoclonal antibodies(mAbs)were often used to identify important epitopes on the antigen in order to ensure the effectiveness of the vaccine.Researches had shown some mAbs that specifically bind to native PT could not recognize the chemically detoxified PT,suggesting that chemical detoxification can change some epitopes of PT,which might influence the protection provided by aPV.Thus we reviewed the researches based on mAbs against PT and summarized the molecular mechanism from antibody interference with PT activities to help understand the related protective immunity after pertussis infection and further develop humanized anti-PT mAbs.
作者
张雨笑(综述)
谌志筠(审校)
何秋水(审校)
ZHANG Yu-xiao;CHEN Zhi-yun;HE Qiu-shui(Department of Medical Microbiology,School of Basic Medical Sciences,Capital Medical University,Beijing 100069,China;不详)
出处
《微生物学免疫学进展》
2020年第5期64-68,共5页
Progress In Microbiology and Immunology
基金
北京市自然科学基金-海淀原始创新联合基金前沿项目(19L2043)
国家“十三五”重大传染病防治科技重大专项(2017ZX10202101-004-001)
关键词
百日咳
百日咳毒素
单抗
抗原表位
疫苗
Pertussis
Pertussis toxin
Monoclonal antibody
Epitope
Vaccine
