摘要
目的主要通过研究胃窦平滑肌M2和M3型胆碱能受体(muscariniccholinergic receptor 2 and 3,M2R/M3R)表达,探讨脾阳虚胃失通降的可能发生机制。方法16只SD大鼠随机分为正常组和脾阳虚组(模型组),每组8只;炭末推进法观察胃残留率;ELISA法检测胃窦平滑肌乙酰胆碱(acetylcholine,ACh)、环-磷酸腺苷(cycle monophosphate,cAMP)和蛋白激酶A(protein kinase A,PKA)含量;免疫组化法检测M2R和M3R表达。结果与正常组比较,模型组胃残留率和ACh水平上升,M2R和M3R表达及cAMP、PKA水平下降,均有统计学意义。结果胃窦平滑肌M2R和M3R表达下调所导致的胆碱能系统功能异常与脾阳虚胃失通降有关。
Objective To study expressions of muscarinic 2 and 3 cholinergic receptors(M2 R/M3 R)on smooth muscles in gastric antrum,attempting to explore the initial mechanism of stomach losing dropping in in spleen yang deficiency.Methods Sixteen SD male rats were randomly divided into the normalgroup and spleen Yang deficiency group(model group)with 8 in each group.The carbon granule propulsion method was used to observe stomach residual rate.ELISA was utilized to measure concentrations of acetylcholine(Ach),cycle monophosphate(cAMP)and proteinkinase A(PKA)in gastric antrum.The immuohistochemistry was used to detect expressions of M2 R and M3 R.Results Compared with those in the normal group,stomach residual rate and Ach level were significantly increased.The expressions of M2 R and M3 R and levels of cAMP and PKA were all decreased markedlyin the model group.Conclusion The abnormality of cholinergic system caused by down-regulation of expressions of M2 R and M3 R in gastric antrum might contribute to stomach failing to descend in spleen Yang deficiency.
作者
王湃
刘文俊
刘旭东
柴纪严
赵金茹
王守岩
王德山
单德红
WANG Pai;LIU Wenjun;LIU Xudong;CAI Jiyan;ZHAO Jinru;WANG Shouyan;WANG Deshan;SHAN Dehong(Xinglin College of Liaoning University of Traditional Chinese Medicine,Shenyang 110167,Liaoning,China;Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China)
出处
《辽宁中医杂志》
CAS
2020年第8期176-178,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家重点基础研究发展计划(2013CB531702)
关键词
脾阳虚
胃主通降
M型受体
乙酰胆碱
迷走神经
spleen Yang deficiency
stomach governing descending
muscarinic receptor
acetylcholine
vagus
