摘要
目的:研究花椒渣中黄酮类成分的含量和抗抑郁作用,为花椒渣的充分利用提供依据。方法:以芦丁为对照品,建立紫外分光光度法测定花椒渣中总黄酮含量的方法,并从花椒渣中提取总黄酮,测定花椒渣和花椒渣提取物中总黄酮的含量。通过小鼠自主活动测试、悬尾实验和强迫游泳实验,测定小鼠血清中的丙二醛(MDA)、超氧化物歧化酶(SOD)和总抗氧化水平,评价总黄酮的抗抑郁作用。结果:紫外分光光度法的加样回收率为98.88%(n=6),花椒渣及其总黄酮提取物中黄酮的含量分别为11.07%和78.42%。与空白组相比,在小鼠自主活动实验中,阳性药组小鼠的站立次数显著增加(p<0.05),总黄酮提取物低、中、高剂量组无显著性差异(p>0.05),各组小鼠活动次数无显著性差异(p>0.05);在小鼠悬尾和强迫游泳实验中,总黄酮提取物高剂量组小鼠不动时间均显著缩短(p<0.05),总黄酮低、中、高剂量组小鼠血清中SOD水平显著升高(p<0.05),总黄酮中、高剂量组小鼠血清中MDA水平显著降低(p<0.05),总抗氧化能力显著提升(p<0.05)。结论:建立的紫外分光光度法能准确测定花椒渣中总黄酮的含量;花椒渣中黄酮类成分丰富,具有显著的抗抑郁作用,其作用机制可能与清除体内自由基和抑制脂质过氧化有关。
Objective:To study the content and anti-depressant effects of the flavonoids in the Zanthoxylum Pericarpium Residue(ZPR),in order to provide a basis for the full utilization of ZPR.Method:The method for the assay of total flavonoids in ZPR by UV spectrophotometry was eatablished with rutin as reference.The total flavonoids were extracted from ZPR,and the contents of total flavonoids in ZPR and its extract were detected.The autonomous activity test,tail suspension test and forced swimming test were conducted in mice,and the levels of malondialdehyde(MDA),superoxide dismutase(SOD)and total antioxidation were detected to evaluate the anti-depressant effects of total flavonoids.Result:The recovery rate of UV spectrophotometry was 98.88%(n=6).The contents of flavonoids in ZPR and its extract were 11.07%and 78.42%,respectively.In the autonomous activity test,compared with the control group,the number of standing times of mice in the positive drug group was significantly increased(p<0.05),but there was not significant difference among the low-,middle-and high-dose groups of total flavonoids extract(p>0.05),as well as there was no significant difference of the activity times among all groups(p>0.05).In the tail suspension and forced swimming tests,compared with the control group,the immobility time of the high-dose group of total flavonoids extract was significantly reduced(p>0.05),and the serum SOD levels of the low-,middle-and high-dose groups were significantly increased(p<0.05).The MDA levels of the middle-and high-dose groups of total flavonoids were significantly decreased(p<0.05),and the total antioxidant capacity was increased significantly(p<0.05).Conclusion:The established UV spectrophotometry can accurately detect the level of total flavonoids in ZPR.ZPR is rich in flavonoids,and has significant anti-depressant effects,which may be related to eliminating internal free radicals and inhibiting lipid peroxidation.
作者
陈文静
杨婷
黄红
王光燕
曾九僧
李莞
吕光华
CHEN Wen-jing;YANG Ting;HUANG Hong;WANG Guang-yan;ZENG Jiu-seng;LI Wan;LU Guang-hua(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,Sichuan;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China;School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,Sichuan)
出处
《中药与临床》
2022年第3期73-77,共5页
Pharmacy and Clinics of Chinese Materia Medica
基金
四川省高校科研创新团队建设计划(18TD0017)
成都中医药大学杏林学者学科人才科研提升计划-中药学学科特色创新科研团队(CXTD2018018)
关键词
花椒渣
黄酮
含量测定
抗抑郁
抗氧化
丙二醛
超氧化物歧化酶
Zanthoxylum Pericarpium Residue
flavonoids
content determination
anti-depressant
antioxidant
malondialdehyde
superoxide dismutase
