摘要
桔梗匍柄霉叶斑病是近年来发现的新病害,病原菌为桔梗匍柄霉Stemphylium platycodontis。为了更好地了解病害的发生规律,本研究对该病原菌的生物学特性及药剂敏感性进行了分析。结果表明:25℃为该病原菌的最适生长温度和产孢温度;菌丝生长最适pH值为4,而最适合产孢的pH值为8;24h黑暗处理,对病原菌的菌丝生长和产孢都最有利;PCA培养基对菌丝生长最有利,而桔梗汁液培养基对病原菌产孢最有利;可溶性淀粉对菌丝生长最有利,而蔗糖最利于病原菌产孢;乙酰铵对病原菌生长及产孢都最有利;26.8μg/mL氟硅唑对桔梗匍柄霉的抑制效果最好,毒力持效性最强。
Stemphylium platycodontis is found to be a pathogen of new disease of Platycodon grandiflorum.The occurrence of the disease and the biological characteristics and fungicide sensitivity of the pathogen were investigated.25°C was optimal for mycelium growth and sporulation;the optimum pH value for mycelium growth was 4,and for sporulation was 8;the dark treatment for 24 h was beneficial to the growth of mycelia and sporulation;PCA medium was the most favorable for the growth of mycelia,and Platycodon grandiflorum seedling juice medium was the most favorable for sporulation.Soluble starch was also favorable for mycelial growth,while sucrose was also favorable for sporulation.Acetylammonium is also found to be beneficial to the growth and sporulation of the pathogen.Fungicide sensitivity observation proved that 26.8μg/mL fluconazole had the best inhibitory effect and the strongest virulence.
作者
徐佳
王燕春
XU Jia;WANG Yan‐Chun(Chifeng Academy of Agricultural and Animal Husbandry Sciences,Chifeng,Inner Mongolia 024031,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2020年第2期312-322,共11页
Mycosystema
基金
内蒙古农牧业科学院青年创新基金项目(2018QNJJNO14).
关键词
桔梗匍柄霉
生物学特性
杀菌剂
毒力持效性
Stemphylium platycodontis
biological characteristics
fungicide
virulence and efficacy
