摘要
目的:通过对瘦素(LEP)基因多态性与宁夏回族人群膝骨性关节炎(OA)的相关性研究,探讨LEP基因与膝OA易感的关系,以进一步了解膝OA发病机制并提供防治相关理论依据。方法:本研究收集148例兼具原发症状和影像证据、不相关的宁夏回族膝OA患者和155例性别、年龄及体质量指数相匹配的对照人群,通过聚合酶链反应和限制性酶切技术,对LEP G2548A位点行单核苷酸多态性(SNP)检测,对基因型分布和等位基因频率分别行拟和优度χ~2检验,并行Hardy-Weinberg平衡检验,Logistic回归分析两个位点不同基因型对膝OA发生风险率的影响。结果:两组间的性别及年龄构成均无统计学差异。经拟合优度χ~2检验结果发现LEP G2548A位点的P>0.05,均符合Hardy-Weinberg遗传平衡。膝OA组LEP G2548A位点AG/GA+AA基因型和A等位基因的分布频率明显高于对照组,LEPR A668G位点AG/GA+GG基因型和G等位基因的分布频率和对照组存在差异(P=0.047和P=0.017)。结论:LEP G2548A位点的多态性与宁夏回族人群中膝OA易感性相关,有利于宁夏回族膝OA发病的危险预测和早期防治。
Objective:To investigate the association of leptin(LEP)gene polymorphism with knee osteoarthritis(OA)susceptibility among the Ningxia Hui population and identify the relationship between the gene polymorphisms and knee OA;To further understand the pathogenesis of knee OA and to provide theoretical basis for early prevention and effective prevention and control.Methods:A case-control study has been adopted,148 identified and un-related knee OA cases were selected from Ningxia Hui population,another 155 cases of gender,age,and body mass index matched subjects were selected as control group.The polymerase chain reaction and restriction fragment length polymorphism(PCRRFLP)analysis were performed to investigate the single nucleotide polymorphism(SNP)of LEP G2548 A site;parallel Hardy-Weinberg balance and Logistic regression analysis was adopted to evaluate the relationship between the genotypes and knee OA in Ningxia Hui population.Results:No difference in the constituent ratios of gender and age were found between the OA group and control group.The goodness of fit chi-square test showed that LEP G2548 A SNP P value is greater than0.05,which conforms to the Hardy-Weinberg balance.LEP G2548 A site AG/GA+AA genotype and allele frequency of the distribution of A in knee OA group were significantly higher than in control group(P=0.047 and P=0.017).Conclusion:LEP G2548 A polymorphism is associated with susceptibility to knee OA in Ningxia Hui population,and it could be used in predicting the risk of knee OA and in early control after prevention.
作者
马晓军
沈佳
罗小军
马甲升
金群华
MA Xiaojun;SHEN Jia;LUO Xiaojun;MA Jiasheng;JIN Qunhua(People's Hospital of Ningxia Hui Autonomous Region,Yinchuan 750000,Ningxia,China;The First Affiliated Hospital of Northwest University for Nationalities,Yinchuan 750000,Ningxia,China;People's Hospital of Autonomous Region Affiliated to Ningxia Medical University,Yinchuan 750000,Ningxia,China;General Hospital of Ningxia Medical University,Yinchuan 750004,Ningxia,China)
出处
《武汉大学学报（医学版）》
CAS
2020年第4期637-640,683,共5页
Medical Journal of Wuhan University
基金
宁夏回族自治区重点研发计划一般项目(编号:2018BEG03055)
关键词
瘦素
病例对照研究
单核苷酸多态性
骨性关节炎
回族
Leptin
Single Nucleotide Polymorphism
Case-Control Study
Osteoarthritis
Hui Population
