摘要
目的对山羊离体椎体分组进行不同数量打孔,使用二胺四乙酸二钠(EDTA-2Na)进行脱钙,并对各组椎体脱钙前、脱钙后生物力学测定,寻求一种快速、有效的骨质疏松模型建立方法。方法取20只年龄6~8年的雌性陕南白山羊腰段L1~L5椎体100个,分为A、B、C、D、E五组(每组20个),A组不打孔,B组、C组、D组、E组分别对椎体终板表面垂直方向进行钻10个孔、20个孔、30个孔、40个孔,各组取均10个椎体进行生物力学测试,对比各组间极限抵抗强度及刚度。各组剩余10个椎体测量骨密度后均使用0.4916 mmol/L的EDTA-2Na溶液浸泡,每5 d测量1次各组的骨密度变化,直至各组椎体达到骨质疏松标准停止,记录所需时间对比各组脱钙前骨密度及脱钙后骨密度。脱钙完成后对各组骨质疏松椎体进行生物力学试验,对比各组间极限抵抗强度及刚度。结果(1)浸泡前椎体生物力学实验结果:A、B、C、D、E五组浸泡前的抵抗极限强度及刚度的差异有统计学意义(P<0.001),A、B、C三组相互间的差异无统计学意义(P>0.05),D、E两组间的差异无统计学意义(P>0.05),而D组与A、B、C三组间的差异有统计学意义(P<0.001),E组与A、B、C三组间的差异有统计学意义(P<0.001),可认为钻孔数≤20个在脱钙前对椎体生物力学指标影响不大。(2)椎体骨质疏松模型建立结果:5组间的脱钙前骨密度的差异无统计学意义(P>0.05),脱钙后5组骨密度的差异无统计学意义(P>0.05),可认为钻孔数与所需建模时间呈负相关性。(3)骨质疏松椎体生物力学实验结果:以A组为标准进行两两对比,发现A、B两组相互间的差异无统计学意义(P>0.05),而C组与A、B、D、E四组间的差异有统计学意义(P<0.01),D组与、A、B、C、E四组间的差异有统计学意义(P<0.01),E组与、A、B、C、D四组间的差异有统计学意义(P<0.001)。结论对椎体终板垂直方向钻孔数量为10个时,可在35 d内建立出椎体骨质疏松模型,且不会对椎体的生物力学指标产生明显影响,相比传统不钻孔建模所需的45 d,能明显缩短时间。
Objective To establish a rapid and effective method in construction of osteoporosis model of vertebral body.Methods The 100 lumbar vertebrae(L1-L5)of 20 female goats ages from 6 to 8 years were collected and divided into Group A,B,C,D and E.No endplate hole was drilled in Group A;while 10,20,30 and 40 endplate holes were drilled in Group B,C,D and E,respectively.Biomechanical tests were applied on 10 vertebrae of each group,so as to compare the ultimate resistance strength and stiffness.The other 10 vertebrae of each group were soaked with EDTA-2 Na solution after the measurement of BMD,and the BMD changes of each group were measured every 5 days until the BMD reached the standard of osteoporosis.The modeling time was recorded for comparison.After decalcification,biomechanical tests were performed on the osteoporotic vertebral bodies of each group to compare the ultimate resistance strength and stiffness.Results Before decalcification,there were significant difference in ultimate strength and stiffness among the 5 groups(P<0.001);and the significant differences were revealed only between Group D and Group A,B or C;and between Group E and Group A,B and C(P<0.001).This suggested that endplate hole number≤20 has little influence on the biomechanical indexes of vertebral body before decalcification.There was no significant difference in bone mineral density among the five groups before decalcification(P>0.05),neither after decalcification(P>0.05).There was no significant difference in BMD between Group A and B(P>0.05).There were significant differences in BMD between Group C and Group A,B,C or D(P<0.01),while significant differences were also revealed between Group D and Group A,B,C or D(P<0.001).Conclusion With 10 drilling holes in vertebral endplate,the vertebral osteoporosis model can be established within 35 days without significant impact on the biomechanical indexes of the vertebral body.Compared with the traditional 45 days without drilling,the time can be significantly shortened.
作者
冯庆裕
谢文伟
王志坤
钟欢
张家勋
FENG Qing-yu;XIE Wen-wei;WANG Zhi-kun;ZHONG Huan;ZHANG Jia-xun(Graduate School of Guangdong Medical University,Zhanjiang 524000,Guangdong,China)
出处
《广东医学》
CAS
2020年第2期118-122,共5页
Guangdong Medical Journal
基金
东莞市社会科技发展(重点)项目(201850725024063).
