摘要
目的对青海省同仁县喜马拉雅旱獭血清中鼠疫耶尔森菌(Yersinia pestis)相关抗体进行检测,为鼠疫流行病学调查和鼠疫菌在自然界保存机制研究提供参考依据.方法以2021年青海省同仁县采集的200份喜马拉雅旱獭血清为样本,利用鼠疫间接血凝试验检测旱獭血清中鼠疫菌F1抗体;以青藏高原鼠疫疫源地分离的1株野生型鼠疫噬菌体476和实验室诊断用鼠疫噬菌体作为抗原,利用微量板法和双层琼脂平板法检测旱獭血清中的特异性鼠疫噬菌体免疫抗体.结果喜马拉雅旱獭血清中鼠疫菌F1抗体检测结果为阴性;2株鼠疫噬菌体分别与200份喜马拉雅旱獭血清进行中和试验,通过点滴法均未检测到与鼠疫噬菌体抗原反应的特异性噬菌体免疫抗体.结论青海省同仁县喜马拉雅旱獭血清中既未发现鼠疫菌F1抗体,也未发现特异性鼠疫噬菌体抗体,二者均间接说明同仁县采样地点的鼠疫疫源地正处于静息期状态.
Objective To detect antibodies related to Yersinia Pestis in the serum of Marmota Himalayana in Tongren county,Qinghai Province,in order to provide reference for the epidemiological investigation of plague and the conservation mechanism of Yersinia pestis in nature.Methods 200 Marmots were collected from Tongren County,Qinghai Province in 2021,and plague indirect hemagglutination test was used to detect yersinia pestis F1 antibody in marmots.A wild-type plague phage 476 isolated from the plague focus on the Qinghai-Tibet Plateau and the plague phage used for laboratory diagnosis were used as antigen.The specific immune antibody of plague phage in Marmots serum was detected by microplate method and double AGAR plate method.Results The serum antibody of Yersinia pestis F1 in M.himalayana was negative.Two strains of plague phage were neutralized with 200 M.himalayana sera,and no specific phage immune antibody was detected by drip method.Conclusion No F1 antibody or Y.pestis bacteriophage specific antibody was found in the serum of M.himalayana in Tongren County,Qinghai Province,both of which indirectly indicated that the plague focus at the sampling site in Tongren County was in a resting state.
作者
张晓璐
李广辉
马龙
杨建国
游陪松
李翔
金泳
李胜
冯建萍
李存香
ZHANG Xiaolu;LI Guanghui;MA Long;YANG Jianguo;YOU Peisong;LI Xiang;JIN Yong;LI Sheng;FENG Jianping;LI Cunxiang(Qinghai Institute for Endemic Diseases Control and Prevention,Xining,Qinghai 810021,China)
出处
《中华卫生杀虫药械》
CAS
2023年第6期539-543,共5页
Chinese Journal of Hygienic Insecticides and Equipments
基金
青海省医药卫生科技项目重点课题(2022-wjzd-06)
关键词
喜马拉雅旱獭
鼠疫噬菌体
鼠疫抗体
流行病学
Marmota himalayana
Yersinia pestis bacteriophage
plague antibodies
epidemiology
