摘要
目的建立气相色谱-串联质谱法同时测定葱中咪鲜胺及其代谢物2,4,6-三氯苯酚的含量。方法样品经均质、提取、净化后,利用气相色谱-串联质谱法在多反应离子监测模式下对溶液进行检测,外标法定量。结果咪鲜胺和2,4,6-三氯苯酚在0.05~0.50 mg/L的质量浓度范围内线性关系良好,相关系数r^(2)在0.999以上;咪鲜胺和2,4,6-三氯苯酚的检出限均为0.004 mg/kg。在0.1、0.3、0.5 mg/kg 3个不同加标水平下,咪鲜胺平均回收率为82.7%~107.4%;2,4,6-三氯苯酚平均回收率为85.4%~109.8%。相对标准偏差为3.8%~9.7%。结论该方法准确、快速,满足大批量葱中咪鲜胺及其代谢物的检测要求。
Objective To establish a method for the simultaneous determination of imidamine and its metabolite 2,4,6-trichlorophenol in onion by gas chromatography-tandem mass spectrometry.Methods The samples were homogenized,extracted and purified,and the solution was detected by gas chromatography-tandem mass spectrometry in multiple reactive ion monitoring mode,and quantitative by external standard method.Results The linear relationship between imidamine and 2,4,6-trichlorophenol was good in the concentration range of 0.05‒0.50 mg/L,and the correlation coefficient r^(2) was above 0.999.The limits of detection of imidamine and 2,4,6-trichlorophenol were 0.004 mg/kg.The average recoveries of imidamine were 82.7%‒107.4%under 3 different supplemental levels of 0.1,0.3 and 0.5 mg/kg,and the average recoveries of 2,4,6-trichlorophenol were 85.4%‒109.8%.The relative standard deviations were 3.8%‒9.7%.Conclusion The method is accurate and rapid,and can meet the requirements for the detection of imidamine and its metabolites in large quantities of scallions.
作者
胡文彬
魏琴芳
叶琼环
洪琼雨
朱锦雨
邢峰
HU Wen-Bin;WEI Qin-Fang;YE Qiong-Huan;HONG Qiong-Yu;ZHU Jin-Yu;XING Feng(Zhejiang Fangyuan Test Group Co.,Ltd.,Hangzhou 310000,China)
出处
《实验室检测》
2023年第8期20-25,共6页
Laboratory Testing
关键词
气相色谱-串联质谱法
咪鲜胺
2
4
6-三氯苯酚
葱
gas chromatography-mass spectrometry
prochloraz
2,4,6-trichlorophenol
green onion
