摘要
Objective:To evaluate the antimicrobial potential of partially purified leaf,bark and root extracts obtained from two mangrove species Rhizophora apiculata and Bruguiera gymnorrhiza from South Andaman against clinical bacterial and fungal pathogens.Methods:Roots,bark and leaves were dried in the shade and subjected to organic solvent extraction.Antibacterial and antifungal activities were performed by agar well diffusion technique.Column purified extracts were analyzed by high performance liquid chromatography for compound identification.Results:Results of the partially purified extracts were analyzed by column chromatography.Fractions collected by high performance liquid chromatography exhibited a wide range of antimicrobial activities against several bacterial and fungal pathogens.Fungal pathogen Aspergillus niger(25 mm)was found to be more sensitive against the mangrove extracts as compared with Klebsiella pneumoniae(23 mm),Escherichia coli,Shigella flexneri,Salmonella typhi(22 mm).Active fractions were identified as tannin compounds based on the peaks obtained by high performance liquid chromatography.Conclusions:Present findings reveal that mangrove bark,roots,and leaves contain valuable metabolites,which have significant importance in the pharmacological industries.Hence,this study suggests that these two mangrove plants Rhizophora apiculata and Bruguiera gymnorrhiza are potential candidates for discovering antimicrobial compounds against clinical pathogens.
