摘要
根据野外采集的獐肌肉、皮张、毛发、血迹、骨骼和粪便等不同样本的特点采用相适合的DNA提取方法,并对肌肉、皮张、毛发、骨骼的提取进行了改进,通过对线粒体细胞色素b和控制区基因PCR扩增反应以及测序结果证实,这几种DNA抽提方法及相应改进的可靠性,并可以提高野外非损伤性取材在保护遗传学中的应用。
Muscle,fur,blood spots,bone,and feces of Chinese water deer for genetic research were collected.Several DNA extraction methods were improved and applied to muscle,fur,blood spots,and bone.Mitochondrial DNA cytochrome b and D-loop gene were amplified and sequenced successfully from those DNA,suggesting that the methods of DNA extraction were reliable,efficient and with extend value to noninvasive samples in conservation genetics study.
出处
《四川动物》
CSCD
北大核心
2006年第3期481-484,共4页
Sichuan Journal of Zoology
关键词
獐
DNA提取
保护遗传学
Chinese water deer
DNA extraction
conservation genetics
