摘要
目的:观察益气明目口服液灌胃对大鼠实验性高眼压所致视网膜缺血再灌注(RIR)诱发视网膜损害的防护作用。方法:SD大鼠随机分为正常组、模型组、低剂量组、高剂量组。应用前房恒压灌注液体升高眼内压的方法建立大鼠R IR模型。低、高剂量组于造模前7 d分别给予不同剂量的益气明目口服液灌胃给药,正常组及模型组给予等量生理盐水灌胃。分别于缺血再灌注损伤后1、3、7、15 d检测视网膜组织中超氧化物歧化酶(SOD)的活性及丙二醛(MDA)的含量,并进行视网膜透视电子显微镜和光学显微镜的组织学观察。结果:缺血再灌注损伤后3、7、15 d,益气明目口服液低、高剂量组视网膜中SOD的活性均高于模型组,益气明目口服液高剂量组MDA的含量低于模型组;通过视网膜透视电子显微镜和光学显微镜的组织学观察,益气明目口服液低、高剂量组的病理组织学损害较模型组明显减轻。结论:益气明目口服液对视网膜缺血再灌注损伤有保护作用,是治疗视网膜缺血性疾病的有效中药复方制剂。
Objective: To investigate the protective effect of the Yiqi Mingmu solution irrigated into rat on retinal injury of rat induced by ischemia reperfusion.Methods: SD rats were divided into four groups randomly: negative control group,model control group,low and high dose group.Increasing the intraocular pressure with anterior chambers infused liquid induced the models of retinal ischemia reperfusion of rats.Different doses of Yiqi Mingmu solution were respectively irrigated into rats 7 days before ischemia in...
出处
《中药材》
CAS
CSCD
北大核心
2008年第1期91-94,共4页
Journal of Chinese Medicinal Materials
基金
广东省中医药局课题(102070,1040065)
