摘要
以不同稀释度的H4亚型禽流感病毒(AIV)感染MDCK细胞,分别在添加与不添加TPCK-胰酶的培养基中培养36 h后收集细胞。经固定后,加入抗H4亚型AIV的单抗2C11,继续加入荧光标记的抗鼠Ig抗体,用流式细胞仪(FCM)分析不同条件下病毒感染的细胞,并用血凝(HA)试验检测细胞培养上清中病毒的HA效价。FCM结果表明:不论加TPCK-胰酶与否,均能引起病毒的感染,且随着感染剂量的增加,含有病毒细胞的比率增加。相比而言,加胰酶后,低剂量感染便可引起病毒在MDCK细胞内大量增殖,并能检测到细胞上清中的病毒。FCM与HA试验相比,FCM试验更敏感,更能反应病毒在感染早期的增殖情况。
MDCK cells,which were infected by H4 subtype avian influenza virus with different diluted concentration were cultured in the culture medium containing TPCK-trypsin or not,seperately.36 hours later,cells were fixed and analysed by Flow Cytometry(FCM) after stained with monoclonal antibody 2C11.Simultaneously,the HA test was subjected to detect virus in cell culture.FCM result indicated that the percent of virus-infected cells were detected and consistent to the infectous dose,whether containing TPCKtrypsin o...
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2007年第4期10-12,共3页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
江苏省高校自然科学重大基础研究项目(05KJA23016)
关键词
禽流感病毒
血凝试验
流式细胞术
avian influenza virus
hemagglutinin test
flow cytometry
