摘要
为了研究重组CHO细胞乙肝表面抗原(CHO-rHBsAg)在小鼠中诱导T细胞免疫应答的能力,全面评价疫苗的免疫原性,以CHO-rHBsAg免疫BALB/c小鼠,常规制备小鼠脾脏淋巴细胞并在体外以抗原或特异多肽刺激;采用ELISA法测定抗原特异性T淋巴细胞分泌的细胞因子,乳酸脱氢酶法(LDH)测定抗原特异性细胞毒T淋巴细胞(CTL)活性,酶联斑点法(ELISPOT)测定CTL频数(CTLp),应用流式细胞仪分析T淋巴细胞亚群。结果显示,rHBsAg可在小鼠中诱导Th1及Th2类细胞因子;加铝佐剂的rHBsAg较未加佐剂的抗原可诱导较高水平的IFN-γ、CTL克隆及较高百分比的CD8+T淋巴细胞亚群。重组CHO细胞来源的HBsAg可在BALB/c小鼠中诱导一定程度的细胞免疫应答。
To study the response of T cell-mediated immunity(CMI) primed with CHO cell derived recombinant hepatitis B surface antigen(rHBsAg) in mice and evaluate the immunogenicity of vaccine,BALB/c mice were immunized by rHBsAg with or without adjuvant.Then spleen-lymphocytes were isolated and stimulated respectively by Ag or specific peptide for investigating CMI response.Cytokine assay,specific CTL activity,CTLp assay and the percentage of phenotypes in T cell were detected respectively by ELISA,LDH,ELISPOT and FCM.rHBsAg can trigger Th1 and Th2 type immune response.Comparing with naked rHBsAg,rHBsAg with Al(OH)3 adjuvant induced higher IFN-γ,more CTL clones and higher percentage of CD8+T cell population.CHO cell derived rHBsAg can induce CMI response to a certain extent in BALB/c mice.
出处
《微生物学免疫学进展》
2007年第4期6-9,共4页
Progress In Microbiology and Immunology
