摘要
【目的】研究酵母拮抗菌Cryptococcus laurentii,病原菌Monilinia fructicola与甜樱桃果实在接种伤口处的互作效应。【方法】在‘先锋’甜樱桃果实伤口处分别单独接种病原菌、酵母拮抗菌以及病原菌与酵母拮抗菌共同接种,分析不同处理体系下果实的发病、酵母拮抗菌的生长动态、果实的脂质过氧化以及氧化酶与抗氧化酶活性的变化。【结果】酵母拮抗菌C.laurentii在25和1℃下均能有效抑制甜樱桃果实褐腐病M.fructicola发生。病原菌M.fructicola在接种初期刺激拮抗菌生长,而后期则抑制拮抗菌。单独接种病原菌M.fructicola或同时接种拮抗菌和病原菌均能诱导果实超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性升高并加速脂质过氧化作用。病原菌M.fructicola侵染甜樱桃果实发病后特异诱导多酚氧化酶(PPO)同工酶新酶带出现;而拮抗菌C.laurentii对甜樱桃果实的抗氧化酶体系、脂质过氧化程度以及PPO同工酶谱的作用效果相对较弱。【结论】在酵母拮抗菌C.laurentii、病原菌M.fructicola与甜樱桃果实的相互作用中,甜樱桃果实褐腐病病害发生受到抑制的主要原因是M.fructicola刺激拮抗菌的生长并诱导果实抗性相关酶活性升高。
【Objective】The present study is mainly to investigate the antagonist-pathogen-host interaction in wounds of sweet cherry fruit. 【Method】Sweet cherry fruits (Prunus avivum L. cv. Van) were inoculated with C. laurentii, M. fructicola, or C. laurentii plus M. fructicola to detect the population dynamics of the antagonist, fruit infection rate, lesion diameter as well as the change of lipid peroxidation and activities of antioxidant enzymes.【Result】Antagonistic yeast Cryptococcus laurentii significantly reduced the brown rot of sweet cherry fruit at 25℃ and 1℃ caused by Monilinia fructicola. Populations of the yeast increased faster in the presence of the pathogen at first, but then decreased rapidly. In fruits inoculated with M. fructicola alone or combined with C. laurentii, an induction of lipid peroxidation and activities of antioxidant enzymes, such as SOD, CAT, and POD, was observed. The isoenzyme pattern of PPO changed greatly after the symptoms appeared, with new PPO isoforms being induced. By contrast, the induction of lipid peroxidation and activities of SOD, CAT, and POD was low while no significant change was found in PPO isoenzyme in fruits inoculated with antagonist C. laurentii alone. 【Conclusion】The inhibition of brown rot during the antagonist-pathogen-host interaction in wounds of sweet cherry fruit is mainly due to the stimulated growth of C. laurentii as well asthe induction of anti-oxidant enzymes of fruits by M. fructicola.
出处
《中国农业科学》
CAS
CSCD
北大核心
2007年第12期2811-2820,共10页
Scientia Agricultura Sinica
基金
中国科学院知识创新工程重要方向项目(KSCX2-YW-G-010)
国家自然科学基金项目(300671473)
关键词
罗伦隐球酵母
褐腐病
甜樱桃
互作效应
Cryptococcus laurentii
Monilinia fructicola
Sweet cherry
Interaction
