摘要
采用三氟化硼-甲醇为衍生化试剂,以HP-FFAP毛细管柱为分离柱,建立了培氟沙星的气相色谱分析法.培氟沙星在质量浓度1.0 mg/L^250.0 mg/L内,药物与内标物峰面积比和药物浓度呈良好的线性关系,检出限为10μg/L.该方法前处理步骤简单,处理后杂质干扰少,灵敏度高,适用性强,用于药物制剂和体液中培氟沙星含量的测定,平均回收率为93.75%~102.73%,日间RSD均小于2.85%(n=5).
A new method has been developed for the determination of pefloxacin(PEFX) in pharmaceutical dosage forms and human urine by derivatization gas chromatography.PEFX was esterified with boron trifluoride catalyzed reaction and analyzed by GC-FID with HP-FFAP capillary column(25 m×0.20 mm×0.33 μm).The calibration curve had good linearity in the ranges of 1.0 mg/L^250.0 mg/L,and the correlation coefficient was 0.9989 for PEFX,the detection limit was 10 μg/L.The method has been successfully applied to determine PEFX pharmaceutical dosage forms with good precision and accuracy,and also applied for the determination of studied drugs in human urine.
出处
《山西师范大学学报（自然科学版）》
2006年第4期64-67,共4页
Journal of Shanxi Normal University(Natural Science Edition)
基金
山西省自然科学基金资助项目(20041030)
关键词
气相色谱法
衍生化
三氟化硼
培氟沙星
尿
gas chromatography
derivatization
boron trifluoride
pefloxacin
urine
