摘要
植物标本作为一种非常重要的DNA资源受到越来越多的关注.由于其储存时间久远及储存手段局限,使得植物标本DNA提取和扩增相当困难.本研究以桑科榕属的9个种作为研究对象,采用4种DNA提取方法,比较其产物的数量和质量,并将所提取的DNA作为模版进行PCR扩增ITS和trnL-F片段.通过比较,得出以下结论:①标本的制作和储存条件对于植物标本DNA的质量有重要影响;②改进的CTAB法对于桑科榕属植物的DNA提取最为适合;③DNA模版的质量较之其他因素对PCR扩增的影响更大,适当缩短扩增片段的长度对实验的成功是至关重要的.
Recently herbarium specimens attract more and more attentions as an important DNA resource.It is rather difficult to extract DNA from herbarbium specimens because of their long-term storage.We sampled 9 herbarium specimens of Ficus using 4 DNA extraction methods.Products of the 4 methods were used as templates in PCR amplification for ITS and trnL-F.After comparison,we draw 3 conclusions as follow: 1.the quality of DNA is affected by the facture and depositing situation of the herbarium specimens;2.modified...
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第S1期328-334,共7页
Journal of Yunnan University(Natural Sciences Edition)
基金
中国科学院知识创新方向性项目(KSCX2-SW-123)
