摘要
在38℃和39℃不同温度条件下培养山羊卵母细胞,结果表明:在38℃和39℃下成熟率分别为63.6%和57.3%,二者之间差异不显著,说明只要培养温度在山羊正常体温的变动范围内,对其卵母细胞的成熟率没有影响;在38℃温度条件下用10%FBS的M199培养液成熟率为62.5%,而10mg/mlBSA的M199培养液成熟率为58.7%,二者之间差异不显著,说明无血清培养可以代替血清培养。
At different temperatures 38℃ or 39℃,the maturation rates of Goats Oocytes were 63.6% and 57.3%,respectively.There were no significant differences between two temperatures(P>0.05),The results showed that temperature had no obvious effect on IVM of Goats Oocytes as long as the educated temperature was within the variety scope of the normal body temperature of Goats ;At 38℃,10mg/mlBSA vs 10% FCS,the maturation rates were 58.7% and 62.5%,respectively.There were no significant differences between the group with BAS and with FCS(P>0.05).The results showed that sera in IVM could be replaced.
出处
《中国草食动物》
2006年第1期12-14,共3页
China Herbivores
