摘要
目的分离钩藤碱与异钩藤碱,测定钩藤中的钩藤碱。方法采用高效毛细管电泳法,运行缓冲液为0.05 mol.L-1硼砂溶液(pH6.9)-0.02 mol.L-1羟丙基-β-环糊精和0.05 mo.lL-1胆酸钠的甲醇溶液(85:15),操作电压为12 kV,温度为40℃,检测波长为250 nm。结果钩藤碱0.025~0.496 mg.ml-1与峰面积呈良好的线性关系(r=0.9998),加样回收率为99.8%,RSD=1.91%。结论所用方法可快速有效地分离钩藤碱及异钩藤碱,并测定植物中的钩藤碱。
OBJECTIVE To develop a method for the determination of Rhynchophylline in Uncaria rhynchophylla Jacks and the separation of rhynchophylline and isorhynchophylline.METHODS HPCE was used with the running buffer of 0.05 mol·L-1 sodium tetraborate solution-0.02 mol·L-1 hydroxypropyl-β-cyclodextrin and 0.05 mol·L-1sodium cholate in methanol(85:15).The applied voltage was 12 kV.The column temperature was maintained at 40℃.The electropherogram was detected at 250 nm.RESULTS The linearities were obtained over the r...
出处
《华西药学杂志》
CAS
CSCD
北大核心
2008年第3期355-356,共2页
West China Journal of Pharmaceutical Sciences
