摘要
选高产酶的纳豆杆菌发酵,发酵液离心除菌后加入饱和度为30%硫酸铵去杂,然后加入65%的硫酸铵析出纳豆激酶粗提物,然后将粗提物分别过阴阳离子交换柱和疏水层析柱进行提纯酶。比较其提纯倍数和回收率,得出较好得分离纯化方案为:样品依次经过DEAE-SepharoseFastFlow阴离子层析、CM-SepharoseFastFlow阳离子层析和Penpyl-SepharoseCl-4B疏水层析柱,纳豆激酶最终纯化倍数达到32.2,回收率为13.2%。
In order to obtain high purity nattokinase from the fermentative broth of Bacillus subtilis, some separation and purification techniques were explored. The optimized separation and purification process includes the following steps: first removing cells by centrifugation, then removing the impurity protein by 30% ammonium sulfate, precipitating the crude extracts of nattokinase by 65% ammonium sulfate and finally purifying the crude extracts by DEAE-Sepharose fast flow ion-exchange chromatography, CM-Sepharo...
出处
《现代食品科技》
EI
CAS
2007年第1期17-19,共3页
Modern Food Science and Technology
关键词
纳豆激酶
层析
分离
Nattokinase
Chromatography
Purification
