摘要
目的 研究壳聚糖包衣胰岛素乳酸 羟基乙酸共聚物 (PLGA)纳米粒对胰岛素胃肠道吸收的促进作用。方法 以双乳化法制备了胰岛素PLGA复乳 ,壳聚糖用作稳定剂 ,制备了包衣纳米粒 ;观察了粒子大小、表面形态及Zeta电位 ;测定了包封率 ;考察了体外释药行为 ;以糖尿病大鼠评价降血糖水平。结果 包衣纳米粒粒度分布均匀 ,隐约可见层状结构 ,壳聚糖可改变粒子表面Zeta电位 ,提高包封率 ,降低突释 ;灌服 1 0u·kg-1 包衣纳米粒 ,1 4～ 1 6h降血糖水平显著高于未包衣纳米粒 (P <0 0 5) ,药理相对生物利用度提高到 1 5 4%。
Aim To study chitosan-coated poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) on enhancing gastrointestinal absorption of insulin. Methods Insulin-loaded PLGA multiple emulsions were prepared by a double-emulsion method. Using chitosan as a stabilizer, chitosan-coated PLGA-NPs was prepared. The changes of the morphology, size distribution and Zeta potential of the NPs were examined. The encapsulation efficiency was determined by HPLC. The release behaviors in vitro were assessed, and the hypoglyc...
出处
《药学学报》
CAS
CSCD
北大核心
2003年第6期467-470,共4页
Acta Pharmaceutica Sinica
关键词
壳聚糖
包衣
胰岛素
纳米粒
胃肠道吸收
chitosan
coating
insulin
nanoparticles
gastrointestinal absorption
