摘要
建立用高效液相色谱法测定紫杉醇含量的方法。以贝克曼ODS4.6250mm为色谱柱,乙腈-甲醇(30∶70)为流动相,流速为1.0mL?min。紫杉醇在10-25μg?mL范围内线性良好,平均回收率为99.9%,RSD=0.97%。本法所用材料经济,测定结果准确,精度高。
Objective:To establish an HPLC method for determination of the content of taxol in powder.Method:Backman ODS 4.6250mm chromatographic column was used.The mobile phase was consisted of acetonitrile-methol (30∶70)and the flaw rate was1.0mL ?min.Result:The linearity was obtained over the range of 10-25μg?mL .The average recovery was99.9%,RSD=0.97%.The method is simple,precrise and accurate.
出处
《哈尔滨商业大学学报（自然科学版）》
CAS
2002年第1期123-124,共2页
Journal of Harbin University of Commerce：Natural Sciences Edition
