摘要
目的 :探讨前列腺增生大鼠前列腺的组织形态学改变。 方法 :采用SD大鼠去势后皮下注射丙酸睾酮法复制前列腺增生模型 ,用水取代法测量前列腺体积 ,苏木精 伊红染色观察前列腺增生组织结构 ,同时结合图像分析系统半定量检测前列腺增生内腺体、间质的形态计量学改变 ,并使用逐步引入剔出模型进行多元线性回归分析。结果 :与正常对照组比 ,模型组前列腺体积明显增大 (P <0 .0 1) ,腺腔扩张、间质增多 ;腺体面积、腺体相对总体积、单位体积内腺体平均直径、平均体积、平均表面积均明显增高 (P <0 .0 1) ,腺体数目、腺体数密度、腺体表面积 /腺体体积、腺体平均曲率显著减少 (P <0 .0 5～ 0 .0 1) ,体积密度无差异 ;间质面积明显减少 (P <0 .0 1) ,但间质相对总体积明显增加 (P <0 .0 5 )。回归分析结果 ,前列腺体积与腺体相对总体积和间质相对总体积呈明显正相关 (r分别为 0 .989和 0 .789,P均 <0 .0 0 1) ;前列腺体积与腺体平均体积呈明显正相关 (r =0 .82 4 ,P <0 .0 0 1)。 结论 :雄激素致去势大鼠前列腺增生以腺上皮增生为主 ,表现为腺腔的扩张 。
Objectives: To investigate prostatic histomorphological changes of rat benign prostatic hyperplasia (BPH) models. Methods: After castrated, SD rats were injected subcutaneously testosterone propionate to induce the BPH . Water substitution method was used to measure prostatic volumes. Prostatic tissues were stained by hemoloxylin and eosin, then morphometric changes of glandular and interstitial tissues were semi quantified by image analysis system.Multiple linear regression was adopted to analyze the results. Results: In comparison with normal group, prostatic volumes were significantly enlarged ( P <0.01) with glands expanded and interstitial tissues increased in BPH model. Glandular average diameters, volumes and surface areas in unit volume, as well as glandular circumferences and glandular relative total volumes were all significantly increased ( P <0.01). Glandular counts, density, ratio of glandular surface area to volume. And glandular average curvature were all declined ( P <0.05～0.01), so was interstitial circumference ( P <0.01). But volume density had no changes, and relative total interstitial volumes were obviously increased. Prostatic volume was significantly correlated with glandular relative total volume ( r =0.989, P <0.001) and interstitial relative total volume( r =0.789, P <0.001). Prostatic volume was also correlated significantly with glandular average volume( r =0.789, P <0.001). Conclusions: Action of androgen on rat prostate may mainly lie in glandular epithelial hyperplasia, which manifests enlargement of glandular lumen accompanied by hyperplasia of interstitial tissues.
出处
《中华男科学杂志》
CAS
CSCD
2002年第3期190-193,共4页
National Journal of Andrology
关键词
前列腺增生
形态计量学
丙酸睾酮
大鼠
Benign prostatic hyperplasia
Morphometry
Testosterone propionate
Rat
