摘要
目的 确认杀菌性 /通透性增加蛋白 (bactericidal/permeabilityincreasingprotein ,BPI)作为机体的自然防御武器在人中性粒细胞中的存在状态。 方法 检测猪源BPI与内毒素(lipopolysaccharide ,LPS)的结合能力及其对LPS诱导的肝库普弗细胞分泌TNFα的抑制作用 ,并采用流式细胞仪测定LPS刺激后PMNL中BPI的表达情况。 结果 猪源BPI与内毒素结合能力随着BPI浓度的增加而增加 ,其抑制LPS诱导肝库普弗细胞分泌TNFα的作用呈剂量效应依赖关系。在LPS刺激 30min内 ,BPI在PMNL表面的表达即明显增加 ,但并未立即释放入血。 结论 BPI具有强大的中和内毒素能力 。
Objective To investigate the existential status of BPI as a natural defensive factor in human polymorphonuclear leukocytes(PMNL). Methods The abilities of porcine BPI to combine endotoxin(lipopolysaccharide, LPS) and inhibit the release of TNFα from hepatic Kupffer's cells were examined. And the expression of BPI in PMNL after stimulation by LPS was determined by flow cytometry. Results The ability of porcine BPI to combine LPS was increased along with the increment of BPI concentration. The e...
出处
《中华烧伤杂志》
CAS
CSCD
2001年第2期38-40,共3页
Chinese Journal of Burns
基金
全军九五攻关课题资助! (96L0 42 )
