摘要
建立了苹果、山楂、番茄制品中棒曲霉素总量的提取方法和液相色谱串联质谱确证方法。清汁样品用乙腈稀释后直接过MycoSep228真菌霉素多功能净化柱;浊汁、固体样品用果胶酶水解后,再用乙酸乙酯提取样品中棒曲霉素,浓缩后经MycoSep228柱净化,用AtlantisdC18色谱柱分离,液相色谱串联质谱测定,电喷雾负离子(ESI-)多反应模式下监测;实验结果表明,样品中棒曲霉素浓度在1.0～200μg/kg范围内时与其峰面积成良好线性关系,检测低限为2.0μg/kg,3个加标水平下回收率为76.0%～95.3%,相对标准偏差为5.4%～11.2%。该方法选择性好,灵敏度高,定性可靠,可满足苹果、山楂、番茄制品中棒曲霉素测定的要求。
A method was developed to clean-up and detect total patulin in of apple,hawthorn and tomato products using HPLC-MS/MS.Clear juice was diluted directly with acetonitrile and cleaned-up with MycoSep 228 multifunctional clean-up column.Cloudy juice or solid samples were pre-treated with pectinase,extracted with acetate ethyl,and then cleaned-up with MycoSep 228.Chromatographic separation was performed on a column Atlantis dC18 and determination was achieved by HPLC-MS/MS with ESI-(electronic spray ionizatio...
出处
《食品科学》
CAS
CSCD
北大核心
2009年第4期162-165,共4页
Food Science
基金
国家认证认可监督管理委员会资助项目(2006B384x)
