摘要
将获自海南(prsv-cp hno1～4)、云南(prsv-cp yunnan)和广西(prsv-cp guangxi)的番木瓜环斑病毒株系外壳蛋白(PRSV-CP)基因序列与DQ340771、X97251(来自中国台湾的PRSV株系)以及AY162218、AY231130、DQ374153、S46722、X97251(分别来自泰国、墨西哥、巴西、美国的PRSV株系)等外壳蛋白(CP)基因序列进行比对,在CP基因高保守区设计靶向目标序列进行亚克隆,获得长度为50bp的DNA片段,并通过一步套叠PCR把50bp正向片段和反向片段与拟南芥内含子拼接,成功构建了小hpRNA结构的植物表达载体,为进一步培育安全性好、抗谱广的番木瓜转基因新种质奠定了基础。
Through aligning PRSV-CP genes in China and those in other countries or regions, 50 bp DNA fragment in high conserved region of PRSV-CP gene were amplified from PRSV strains in Hainan, Yunnan and Guangxi. Using one -step overlap extension PCR, the 50 bp sense fragment and antisense fragment of PRSV-CP were spliced with the Arabidopsis thialiana intron fragment and constructed into plant expression vector pBI121 and pCAMBIA3300. This study could facilitate to obtaining of new transgenic papaya varieties with...
出处
《热带作物学报》
CSCD
2009年第2期176-180,共5页
Chinese Journal of Tropical Crops
基金
国家自然科学基金项目(No.30760134)
中央级科研院所基本科研业务费项目资助。
