摘要
以23份龙舌兰麻种质的嫩叶为材料,采用聚丙烯酰胺垂直板凝胶电泳,分析它们的过氧化物(POD)同工酶酶谱特征,为杂交育种亲本选择提供依据.结果表明,23份材料显示出3～10条不等的酶带,有22种不同的酶谱类型,说明过氧化物同工酶酶谱可鉴定除粤西117和东292之外的21份龙舌兰麻种质;Rf为0.37的酶带为所有供试材料的共有谱带.DPS聚类分析结果表明,23份材料根据亲缘关系远近可分为3大类:第一类为包括金边弧叶龙舌兰、弧叶龙舌兰等在内的9份种质;第二类为包括多叶普通剑麻、南亚2号等在内的5份种质;第三类为包括假菠萝麻、银边假菠萝麻等在内的9份种质.
The young leaves of 23 agave germplasm were used as materials.The peroxidase isozyme(POD)of them were analysed by plumb plate of polyarclamide gel electrophoresis in order to know their relationships.The results of electrophoresis showed there are 3 to 10 bands in different germplasm and 22 zymogram types,which indicated that POD isozyme could be used to identify 21 agave germplasm except Yuexi No.117 and Dong 292 .The band of Rf 0.37 was characteristic of 23 agave germplasm.DPS cluster analysis indicate...
出处
《西北植物学报》
CAS
CSCD
北大核心
2009年第2期253-257,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
中国热带农业科学院中央级公益性科研院所基本科研业务费专项基金(sscri200716)
