摘要
利用不同棉花受体材料进行花粉管通道法遗传转化研究。通过卡那霉素鉴定、抗虫性鉴定和PCR分子生物学鉴定,证明已成功将Bt基因转化到泗棉3号、辽棉15、中棉所19、中棉所29母本、中棉所35、中棉所36等棉花材料中。通过对转基因材料后代与非转基因材料的铃重、衣分和纤维品质等差异分析,发现花粉管通道转基因后代材料存在广泛的变异。
Different cotton lines have been studied in inheritance traits changing by pollen transgenic method.Bt gene has been successfully transformed into Simian 3,Liaomian 15,CCRI 19,CCRI 29,CCRI 35 and CCRI 36 cotton varieties by pollen transgenic method which was correspondingly confirmed by kanamysin judging,insect-resistance detecting,PCR verifying and southern bloting.The SAS analyses indicated that pollen transgenic cotton had large changing in inheritance characteristics with the boll weight,ginning outturn...
出处
《棉花学报》
CSCD
北大核心
2009年第1期23-27,共5页
Cotton Science
基金
"973"计划(2004CB117306)
"十一五"国家科技支撑计划(2006BAD13B04-1-09)
关键词
棉花
花粉管通道
转基因
遗传变异
cotton
pollen-tube pathway
transgenic
genetic variation
