摘要
根据Langendoff心脏灌流系统原理,参照Langendoff心脏灌流系统模型,利用本实验室已有的恒温浴槽和医院常用的静脉输液管组装了蛙心脏灌流系统。利用该灌流系统,采用胶原酶溶液灌流蛙心脏,分离到游离的蛙心肌细胞。为观测蛙心肌细胞通道电生理学特性的实验打下了一定的基础。
According to the principle and model of Langendoff perfusion of the isolated heart,a device for in vitro heart perfusion of frog was prepared with constant temperature trough and the pipe of giving infusion.The device was used to perfuse collagenase into the heart of frog and single myocardium cell was gained.This procedure is helpful to study the electro-physiological property of ion channel in myocardium cell.
出处
《实验室研究与探索》
CAS
北大核心
2009年第1期45-47,共3页
Research and Exploration In Laboratory
基金
南开大学第四届本科生创新科研"百项工程"资助项目
关键词
蛙
心室肌细胞
心脏灌流
frog
myocardium cell
heart perfusion
