摘要
以大肠杆菌表达的猪圆环病毒2型(PCV2)重组衣壳蛋白(His-CAP2)为抗原,通过方阵ELISA滴定,确定了His-CAP2的最适包被浓度为6.4μg·mL^(-1),待检血清稀释度为1:40,建立PCV2的血清流行病学的间接ELISA监测方法。通过对已知PCV2血清及其他病毒血清的试验,表明该ELISA方法具有良好的特异性。利用该方法对来自苏中地区不同猪场的175份血清样本进行检测,PCV2抗体阳性检出率为70.29%,与临床上PCV2感染导致猪群发病的实际情况相符合,证实了此方法临床应用的可行性。
The recombinant capsid protein(His-CAP2)of Porcine circovirus type 2(PCV2) expressed in Escherichia coli BL21(DE3) was used as antigen for developing an indirect-ELISA assay to detect PCV2 specific antibody.Conditions of the ELISA were optimized,and suggested that the optimistic concentration of the recombinant capsid protein was 6.4μg·mL^(-1).Different serum samples were detected for the antibody of PCV2,and it was found that the developed ELISA method was sensitive and specific to the positive serum of PC...
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2009年第1期9-12,共4页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
江苏省“六大人才高峰”第二批项目(2004-11)。
