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胶质瘤中MGMT和hMLH1甲基化与表达水平的变化及意义 被引量：4

The Methylation Status and Expression of MGMT and hMLH1 in Glioma and Their Clinical Significance
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摘要 目的探讨胶质瘤组织中,O6-甲基鸟嘌呤-DNA甲基转移酶(O6-methylguanine DNA methyltransferase,MGMT)和错配修复基因mutLhomolog1(Homo sapiens,hMLH1)的甲基化及蛋白水平的变化,及其在胶质瘤发生中的作用。方法应用甲基化特异性PCR(methylation-specific PCR,MSP)方法检测了78例胶质瘤患者组织中MGMT和hMLH1的启动子区甲基化状态;应用免疫组织化学方法检测其蛋白表达水平。结果MGMT启动子区甲基化43例(55.1%),hMLH1启动子区甲基化32例(41.0%)。MGMT与hMLH1在胶质瘤中表达的阳性细胞百分比的中位数分别为15.9%和84.6%。两种基因的启动子区甲基化状态与蛋白表达的阳性率没有明显的相关性,且均与年龄、性别、病理类型无关。结论胶质瘤细胞中同属于DNA修复系统的MGMT蛋白表达率远低于hMLH1表达率,这提示胶质瘤发生发展过程中可能存在着与DNA修复系统紊乱有关的一些独特的路径。 Objective To explore the methylation status and expression of hMLH1 and MGMT in glioma,and the role of them in the glioma oncogenesis.Methods Methylation status of the MGMT and hMLH1 promoter regions was assayed in 78 glioma tissues by methylation-specific PCR(MSP).The expression of MGMT and hMLH1 in the glioma was investigated by immunohistochemical technique using monoclonal antibody.Results Methylation in the MGMT and hMLH1 promoter regions was found in 43(55.1%)and 32(41.0%)cases respectively.The median...
作者 陈慧媛 江涛 袁芳 崔云 李桂林 黄磊
机构地区 首都医科大学附属北京市神经外科研究所神经病理室 首都医科大学附属北京天坛医院神经外科脑胶质瘤诊治中心
出处 《首都医科大学学报》 CAS 北大核心 2009年第2期203-207,共5页 Journal of Capital Medical University
基金 国家自然科学基金(30772238) 科技部十一五重点课题(2007BAI05B08) 国家自然科学基金重点项目(30730035)资助项目~~
关键词 胶质瘤 MGMT HMLH1 免疫组织化学 甲基化特异性PCR glioma MGMT hMLH1 immunohistochemistry methylation-specific PCR
分类号 R739.4 [医药卫生—肿瘤]
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二级参考文献28

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首都医科大学学报
2009年 第2期

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