摘要
以茎段为外植体,进行龟背竹组培再生技术研究。结果表明,诱导芽分化最佳培养基为1/2MS+BA 2.0 mg.L-1+NAA 0.7 mg.L-1,适宜龟背竹芽增殖的培养基为:1/2MS+BA 1.2mg.L-1+NAA 1.1 mg.L-1+KT 0.3 mg.L-1,增殖系数达4.6;试管苗的生根培养基以1/2MS+NAA 1.0 mg.L-1+0.1%AC组合生根效果最高,生根率达96%。
Using the stems of Monstera deliciosa as explants,the tissue culture and plant regeneration technology was studied.The results showed that the optimum medium for shoot induction was 1/2MS+ BA 2.0 mg.L-1 +NAA 0.7 mg.L-1.The appropriate medium for shoot multiplication was 1/2MS+BA 1.2 mg.L-1+NAA 1.1 mg.L-1+KT 0.3 mg.L-1,in which the propagation coefficient reached 4.6.The rooting medium for plantlets in vitro was 1/2MS+ NAA1.0 mg.L-1 +0.1%AC with the best rooting effect,and the rooting rate reached 96%.
关键词
龟背竹
组织培养
再生体系
Monstera deliciosa
tissue culture
regeneration system
