摘要
目的:制备氟甲砜霉素(FF)的最佳人工抗原并鉴定。方法:采用琥珀酸酐将FF的羟基活化后制备出半抗原氟甲砜霉素半琥珀酸(FF-HS),并经质谱(MS)鉴定合成成功。采用碳二亚胺法将FF-HS与牛血清白蛋白(BSA)偶联,采用混合酸酐法将FF-HS与卵清白蛋白(OVA)偶联,采用羰基二咪唑法将FF直接与BSA偶联,制备了3种人工抗原(FF-HS-BSA、FF-HS-OVA和FF-BSA)。采用紫外吸收光谱分析和动物免疫实验(3种偶联物选择前2种分别作为免疫原与包被原进行抗血清制备及ELISA实验)鉴定3种人工抗原。结果与结论:紫外吸收光谱分析初步证实3种人工抗原合成成功。在动物免疫实验中FF-HS-BSA与FF-HS-OVA得到了效价较高的特异性抗体,IC50分别为150、300μg/L。FF-BSA免疫原免疫效果不理想。
Aim:To prepare and identify florfenicol artificial antigen.Methods:Florfenicol was actived by succinic anhydride and converted into florfenicol succinate as the hapten.The result of MS indicated that the hapten was synthesized successfully.Florfenicol succinate was linked to carrier protein bovine serum albumin(BSA)and ovalbumin(OVA),by carbodiimide method,mixed anhydride method,respectively,and florfenicol was linked to carrier protein BSA as artificial antigen by N,N'-carbonyldiimidazole(CDI)method.Antige...
出处
《郑州大学学报（医学版）》
CAS
北大核心
2009年第6期1166-1168,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省杰出人才创新基金资助项目0421002300
