摘要
目的检测脑缺血及再灌注大鼠血浆中内皮素(ET)和一氧化氮(NO)的浓度变化,研究二者之间的相关性及与肺损伤的关系。方法本实验采用48只wistar雄性大鼠,随机分为8组:正常组、假手术组、脑缺血2h,脑缺血2h再灌注3h、6h、12h、24h、48h组,对各组动物分别用放免法测定血浆中ET、用生化法测定血浆中NO,并取肺组织进行常规组织学观察。结果(1)缺血后2h组血浆NO浓度最低,随着灌注时间的推移,NO浓度逐渐升高,再灌24h及48h后达高峰。缺血2h再灌24h和48h组与正常对照组有显著差异;缺血2h组与其它7组对比均有显著差异;缺血2h再灌24h和缺血2h再灌48h组之间无显著差异,但此2组与其它6组对比均有显著差异;正常组、假手术组、缺血2h再灌3h组、6h组、12h组间均无差异,但它们中的每组与缺血2h组,缺血2h再灌24h组、48h组对比,均有显著性差异。(2)缺血后2h组血浆ET浓度最高,随着灌注时间的推移,ET浓度逐渐降低,缺血2h再灌48h组浓度最低。缺血2h组、缺血2h再灌48h组和正常组对比有显著差异,该2组对比有显著差异,且与其它6组对比均有显著性,而正常组、假手术组、缺血2h再灌3h组、6h组、12h组、24组各组之间均无显著性差异。(3)脑缺血再灌注后,血浆中的NO和ET浓度变化呈负相关。(4)缺血2h组可见毛细血管扩张,部分肺泡腔内有红细胞漏出;再灌注3-6h肺泡腔内有浆液及红细胞渗出,部分肺泡内有炎性细胞,支气管腔有脱落上皮细胞;再灌注12h、24h、48h肺组织呈炎症逐渐加重改变:即肺泡内有红细胞,中性粒细胞和淋巴细胞浸润,肺间质的炎性细胞增多,部分支气管萎缩,肺泡腔及支气管腔均有浆液渗出,肺大泡形成增加。结论(1)随着脑缺血及再灌注的进行,肺损伤逐渐加重;(2)ET和NO在脑缺血再灌注中以不同形式介导了肺损伤;(3)脑缺血再灌注后血浆中ET和NO浓度的变化呈负相关,P<0.01,r=0.592。
Objective To detect the concentration of ET and NO in the blood of reperfused rats of cerebral ischemia and to study the relations between ET.NO and lung injury and whether there exist corelations between ET and NO.Methods 48 wistar male rats were randomly divided into 8 groups:control group false operation group, group of cerebral ischemia for 2 hours ,groups reperfused for 3 hours, 6 hours,12 hours,24 hours,48hours respeetively after 2 hours' cerebral ischemia. The concentrations of ET and NO were measrued in blood plasma of all groups of rats by radio-immunity and by bio-chemistry respectively,and lung tissues were extracted to be corried routine histology observation.Results (1)The concentration of NO was the lowest in group of cerebral inchemia for 2 hours.And with the passage of time perfused,the NO concentration was becoming increasingly high,and the highest when perfused for 48 hours.there were significant differences between that of confrol group and those of groups reperfused for 24 hours and for 48 hours;there were significant differences between the group of ischemia for 2 hours and the other 7 groups;there were no significant differences between the groups reperfused for 24 hours and for 48 hours after ischemia for 2horus,but there were signifcant differences between these 2 groups and the other 6 groups,There was no difference between the control group false operating group and groups reperfused for 3 hours,6 hours,12hours and there were significant differences when compared with those of group of ischemia for 2 hours and groups reperfused for 24 hours, 48hours after 2 hours' ischemia respectively.(2)The comcentration of ET was the highest in group of cerebral ischemia for 2 hours.And with the passage of time perfused,the ET concentration was becoming increasingly low, and the lowest when perfased for 48 hours . There were significant differences between that of control group and those of groups reperfused for 24 hours and for 48 hours; there were significant differences between either of the two groups and the other 6 groups;and there was significant difference between the two groups;there were no significant differences between the control group, false operating group nad groups reperfused for 3 hours,6 hours,12 hours,24 hours.(3)There were corelations betweeen NO and ET,(P<0.01,r=0.592),which was negatively related.(4)The capillarectasia was seen in group of inschemia for 2 hours,and there was leakage of RBC in parts of alveoli there was exudation of serum and RBC in alveoli reperfused for 3-6 hours there were inflamation cells in parts of alveoli;there was parthy exuviation in branchial cavity;After being perfused for 12 hours,24hours,48hours,the change of lung tisswes presented that the inflammation was becoming increasingly serious,there was infiltration of RBC,neutrophil and lymphocyte in alveoli;Pulmonary interstitial inflammatory cells increased,part of branchi shrinked,there was exudation of serum in both alveoli and bronchial cavity;bullae of lung also increased.Conclusion (1)With the progress of cerebral ischemia and reperfusin,the lung injury is becooming increaningly serious;(2)ET and NOcontribufe to lung injurty in different fashion.(3)There is negatively corelation between contration of ET and NO. in the blood of reperfused rats of cerebral ischemia.(P<0.01 , r=0.592).
出处
《中国医药导报》
CAS
2005年第24期1-4,15,共5页
China Medical Herald
