摘要
Objective To explore the relationship between power density of He -Ne laser with the growth of fibroblasts in hypertroph ic scar (HS).Methods The cultured fibroblasts in HS were i rradiated with He -Ne laser(Wavelenth 632.8nm),various power densities such as50mW/cm 2 ,100mW/cm 2 and 150mW/cm 2 were respectively adopted once a day for 10minutes.After 1,3and 5times o f He -Ne laser for dose rate irradiation separatedly ,the cell count and cell circle analysis were counted and examined by trypa n blue staining and flow cytometry .Results The amount of cell after 1times of 50mW /cm 2 laser irradiation was markedly more than teh control(P<0.01).The amount of cells after 5times of 100mW/cm 2 ,3and 5times of 150mW/cm 2 laser irradiation was less than the controls(P <0.05).The result of cell circle analysis was corresponded with that of the cell count.Conclusion Both stimualtion and inhibition of He -Ne laser on the growth of scar fibroblasts can be obtained with vario us power densities.Power density of He -Ne laser associates with the effects on the growth of scar fibroblasts.
Objective To explore the relationship of power density of He-Ne laser with the growth of fibroblasts in hypertrophic scar（ HS） .Methods The cultured fibroblasts in HS were irradiated with He-Ne laser（ Wavelength 632.8nm） ,various power densities such as 50mW/cm2, 100mW/cm2 and 150mW/cm2 were respectively adopted once a day for 10 minutes. After 1,3 and 5 times of He-Ne laser for dose rate irradiation separately, the cell count and cell circle analysis were counted and examined by trypan blue staining and flow cytometry. Results The amount of cells after 1 times of 50mW/cm2 laser irraidation was markedly more than the control (P< 0.01). The amount of cells after 5 times of 100mW/cm2, 3 and 5 times of 150mW/cm2 laser irradiation was less than the controls (P< 0.05). The result of cell circle analysis was corresponded with that of the cell count.Conclusion The study suggests that both stimulation and inhibition of He-Ne laser on the growth of scar fibroblasts can be obtained with various power densities. Power density of He-Ne laser associated with the effects on the growth of scar fibroblasts.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
2001年第18期150-151,共2页
Journal of Clinical Rehabilitative Tissue Engineering Research
