摘要
目的:建立反相高效液相色谱法测定盐酸氟西汀胶囊的含量。方法:采用kromasil C18色谱柱(5μm,4.5mm×15mm)为分离柱;流动相为甲醇:0.05mol/L磷酸二氢钾缓冲液(55:45);流速:1mL／min;检测波长:230nm;柱温:室温。结果:盐酸氟西汀在52.5mg/L-315mg/L浓度范围内线性良好(r=0.9994);方法平均回收率为99.66％(RSD=0.98％,n=5);5次测定的精密度0.16％-0.35％。结论:本方法操作简便、快速、准确、灵敏,适用于盐酸氟西汀胶囊的含量测定。
Objective:A rapid RP-HPLC method was established for the determination of fluoxetine in capsules.Methods:Fluoxetine and dizapam (internal standard)were separated on kromasil C18 column.Methanol:0.05mol/L phosphate butter(55:45)as mobile phase;the flow rate was 1mL/min;The wavelength of detection was 230nm.Results:The linear range of fluoxetine was 52.5mg/L-315mg/L (r =0.9994) .The average recovery was 99.66% ,RSD=0.98%,n=5 and the precisions (RSD)for several determination were 0.16%-0.35%,n=5.Conclusion:This method is simple, rapid, accurate and sensitive.It is suitable for the determination of fluoxetine in capsules.
出处
《中国药物应用与监测》
CAS
2004年第3期52-53,共2页
Chinese Journal of Drug Application and Monitoring
