摘要
定点诱变技术可有目的的改变DNA序列中的任何一个特定的碱基,是基因操作的一种基本技术。综述了常见的几种定点诱变方法,并对它们的优缺点进行了对比。
Site-directed mutagenesis is used to introduce desired mutations into target DNA sequences.Site-directed mutagenesis has become the conventional means to operate gene.Some methods of site-directed mutagenesis are reviewed and discussed in the paper.
出处
《山西农业大学学报(自然科学版)》
CAS
2007年第6期124-126,共3页
Journal of Shanxi Agricultural University(Natural Science Edition)
关键词
基因
定点诱变
PCR
Gene
Site-directed mutagenesis
PCR