摘要
目的调查和研究西安地区鲍曼不动杆菌对碳青霉烯类抗生素耐药分子机制和产碳青霉烯酶情况。方法收集西安地区六所三级甲等医院一年间临床分离的非重复鲍曼不动杆菌株146株,采用K-B法进行药敏试验,E-test法检测金属酶,NaCl抑制试验检测OXA型碳青霉烯酶,PCR扩增OXA-23、OXA-58型碳青霉烯酶基因,其阳性产物经双向测序分析。结果筛选出对亚胺培南耐药的鲍曼不动杆菌非重复株15株,14株耐药菌经E-test法鉴定未产金属酶,经NaCl抑制后发现其对亚胺培南的敏感性增加,提示产生OXA型碳青霉烯酶,其中11株PCR扩增blaOXA-23阳性,1株PCR扩增blaOXA-58阳性,NaCl抑制试验与PCR检测结果的一致率达85.7%。结论产OXA型碳青霉烯酶是西安地区该菌对亚胺培南耐药的主要原因,其OXA-58型基因在国内尚属新型碳青霉烯酶基因型;NaCl抑制试验是一种简单、有效、价廉的表型筛选OXA型碳青霉烯酶的新方法。
Objective To investigate the molecular mechanism of carbapenem resistance in the clinical isolates of Acinetobacter baumannii from Xi'an and their profile of carbapenemase production.Methods A total of 146 Acinetobacter baumannii strains were isolated from 6 general hospitals in Xi'an.Antimicrobial susceptibility test was performed for all the strains,followed by detection of imipenem resistance using E-test for metallo-β-lactamase(MBL) and NaCl inhibition test for OXA type carbapenemase.BlaOXA-23 and blaOX...
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第7期1393-1396,共4页
Journal of Southern Medical University
基金
西安市科技创新支撑计划项目(YF07173)
