摘要
目的研究利用基因重组工程技术构建高活性的氨基酰化酶工程菌连续拆分D,L-蛋氨酸。方法氨基酰化酶工程菌经发酵收集高活性的菌体,通过海藻酸钠包埋技术制备固定化酶,连续拆分D,L-蛋氨酸。结果利用基因工程构建的工程菌表达氨基酰化酶,比酶活性大于6000μ/g湿菌体,酶柱比酶活性大于4000μ/g酶,半衰期20d,可连续拆分24d,拆分率达90%,收率达74.5%左右。结论利用基因工程技术构建工程菌表达的氨基酰化酶活性高和拆分率高。
Objective To investigate a method of constructing highly-active amino-acylase engineering bacteria for a continuous decomposition of D,L-methionines. Method Amino-acylase engineering bacteria were fermented and highly-active bacteria thereby were collected through preparations of immobilized enzymes via sodium alginate embedding techniques. Then, the continuous decompositions of D,L-methionines were performed. Result When amino-acylases were expressed with engineering bacteria constructed via gene engineering techniques, the enzyme activities were over 6000μ/g wet thallus; enzyme activities within columns were over 4000μ/g enzyme; half-lifes were 20 days; possibility for a continuous decomposition was of 24 days; decomposition rates reached 90% and production rates were about 74.5%. Conclusion Activities and decomposition rates of amino-acylases expressed with engineering bacteria constructed via gene engineering techniques were high.
出处
《药品评价》
CAS
2004年第4期290-291,244,共3页
Drug Evaluation
关键词
氨基酰化酶
拆分
蛋氨酸
amino-acylase
decomposition
methionin
